A mitochondria-targeting highly specific fluorescent probe for fast sensing of endogenous peroxynitrite in living cells

Wei Shu; Yulong Wu; Shunping Zang; Sa Su; Hao Kang; Jing Jing; Xiaoling Zhang

doi:10.1016/j.snb.2019.127284

A mitochondria-targeting highly specific fluorescent probe for fast sensing of endogenous peroxynitrite in living cells

Wei Shu, Yulong Wu, Shunping Zang, Sa Su, Hao Kang, Jing Jing^*, Xiaoling Zhang

^*Corresponding author for this work

School of Chemistry and Chemical Engineering

Beijing Institute of Technology

Research output: Contribution to journal › Article › peer-review

48 Citations (Scopus)

Abstract

Peroxynitrite (ONOO⁻), an endogenous reactive oxygen species, acts an important role in various physiological and pathological processes. Herein, we designed a mitochondria targeting fluorescent probe MBTBE for sensitive determination of ONOO⁻. MBTBE exhibits rapid response (< 1 min) and high selectivity towards peroxynitrite with distinct color changing (colorless to orange). The fluorescence intensity at 569 nm was linearly correlated with the concentration of ONOO⁻ (0–10 μM) with a detection limit of 16 nM. Additionally, MBTBE possesses good biocompatibility and mitochondria-targeting. Furthermore, MBTBE was successfully applied to monitor fluctuations in ONOO-levels and H₂S-induced ONOO⁻ clearing in HeLa cells, indicating excellent potential applications in bioanalysis analysis.

Original language	English
Article number	127284
Journal	Sensors and Actuators, B: Chemical
Volume	303
DOIs	https://doi.org/10.1016/j.snb.2019.127284
Publication status	Published - 15 Jan 2020

Keywords

Benzothiazole derived
Bioimaging
Fluorescent probe
Mitochondria
Peroxynitrite

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10.1016/j.snb.2019.127284

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title = "A mitochondria-targeting highly specific fluorescent probe for fast sensing of endogenous peroxynitrite in living cells",

abstract = "Peroxynitrite (ONOO−), an endogenous reactive oxygen species, acts an important role in various physiological and pathological processes. Herein, we designed a mitochondria targeting fluorescent probe MBTBE for sensitive determination of ONOO−. MBTBE exhibits rapid response (< 1 min) and high selectivity towards peroxynitrite with distinct color changing (colorless to orange). The fluorescence intensity at 569 nm was linearly correlated with the concentration of ONOO− (0–10 μM) with a detection limit of 16 nM. Additionally, MBTBE possesses good biocompatibility and mitochondria-targeting. Furthermore, MBTBE was successfully applied to monitor fluctuations in ONOO-levels and H2S-induced ONOO− clearing in HeLa cells, indicating excellent potential applications in bioanalysis analysis.",

keywords = "Benzothiazole derived, Bioimaging, Fluorescent probe, Mitochondria, Peroxynitrite",

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AU - Shu, Wei

AU - Wu, Yulong

AU - Zang, Shunping

AU - Su, Sa

AU - Kang, Hao

AU - Jing, Jing

AU - Zhang, Xiaoling

PY - 2020/1/15

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AB - Peroxynitrite (ONOO−), an endogenous reactive oxygen species, acts an important role in various physiological and pathological processes. Herein, we designed a mitochondria targeting fluorescent probe MBTBE for sensitive determination of ONOO−. MBTBE exhibits rapid response (< 1 min) and high selectivity towards peroxynitrite with distinct color changing (colorless to orange). The fluorescence intensity at 569 nm was linearly correlated with the concentration of ONOO− (0–10 μM) with a detection limit of 16 nM. Additionally, MBTBE possesses good biocompatibility and mitochondria-targeting. Furthermore, MBTBE was successfully applied to monitor fluctuations in ONOO-levels and H2S-induced ONOO− clearing in HeLa cells, indicating excellent potential applications in bioanalysis analysis.

KW - Benzothiazole derived

KW - Bioimaging

KW - Fluorescent probe

KW - Mitochondria

KW - Peroxynitrite

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A mitochondria-targeting highly specific fluorescent probe for fast sensing of endogenous peroxynitrite in living cells

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Keywords

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