A mitochondria-targeted far red fluorescent probe for ratiometric imaging of endogenous peroxynitrite

Wei Shu; Yulong Wu; Tianjiao Shen; Jie Cui; Hao Kang; Jing Jing; Xiaoling Zhang

doi:10.1016/j.dyepig.2019.107609

A mitochondria-targeted far red fluorescent probe for ratiometric imaging of endogenous peroxynitrite

Wei Shu, Yulong Wu, Tianjiao Shen, Jie Cui, Hao Kang, Jing Jing^*, Xiaoling Zhang

^*Corresponding author for this work

School of Chemistry and Chemical Engineering

Beijing Institute of Technology

Research output: Contribution to journal › Article › peer-review

40 Citations (Scopus)

Abstract

Peroxynitrite (ONOO⁻), one reactive oxygen species, is related to various physiological and pathological activities. However, detailed mechanisms of peroxynitrite in biological systems are not thoroughly understood. Thus, the accurate detection of peroxynitrite with rapid response is essential for revealing its functions. Herein, we present a far red fluorescent probe Mito-WQPhOH to ratiometric monitor ONOO⁻ in vitro and living cells. Mito-WQPhOH showed the merits of fast response (<20 s), outstanding selectivity and excellent sensitivity towards ONOO⁻. Importantly, owing to the good water-solubility, great biocompatibility and excellent mitochondria targeting of Mito-WQPhOH, it was used for ratiometric imaging endogenous ONOO⁻ in living macrophages. Therefore, Mito-WQPhOH could act as a potential tool to reveal mechanisms of ONOO⁻ in biological systems.

Original language	English
Article number	107609
Journal	Dyes and Pigments
Volume	170
DOIs	https://doi.org/10.1016/j.dyepig.2019.107609
Publication status	Published - Nov 2019

Keywords

Bioimaging
Fluorescent probe
Mitochondria
Peroxynitrite
Ratiometric determination

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10.1016/j.dyepig.2019.107609

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title = "A mitochondria-targeted far red fluorescent probe for ratiometric imaging of endogenous peroxynitrite",

abstract = "Peroxynitrite (ONOO−), one reactive oxygen species, is related to various physiological and pathological activities. However, detailed mechanisms of peroxynitrite in biological systems are not thoroughly understood. Thus, the accurate detection of peroxynitrite with rapid response is essential for revealing its functions. Herein, we present a far red fluorescent probe Mito-WQPhOH to ratiometric monitor ONOO− in vitro and living cells. Mito-WQPhOH showed the merits of fast response (<20 s), outstanding selectivity and excellent sensitivity towards ONOO−. Importantly, owing to the good water-solubility, great biocompatibility and excellent mitochondria targeting of Mito-WQPhOH, it was used for ratiometric imaging endogenous ONOO− in living macrophages. Therefore, Mito-WQPhOH could act as a potential tool to reveal mechanisms of ONOO− in biological systems.",

keywords = "Bioimaging, Fluorescent probe, Mitochondria, Peroxynitrite, Ratiometric determination",

author = "Wei Shu and Yulong Wu and Tianjiao Shen and Jie Cui and Hao Kang and Jing Jing and Xiaoling Zhang",

note = "Publisher Copyright: {\textcopyright} 2019 Elsevier Ltd",

year = "2019",

month = nov,

doi = "10.1016/j.dyepig.2019.107609",

language = "English",

volume = "170",

journal = "Dyes and Pigments",

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TY - JOUR

T1 - A mitochondria-targeted far red fluorescent probe for ratiometric imaging of endogenous peroxynitrite

AU - Shu, Wei

AU - Wu, Yulong

AU - Shen, Tianjiao

AU - Cui, Jie

AU - Kang, Hao

AU - Jing, Jing

AU - Zhang, Xiaoling

PY - 2019/11

Y1 - 2019/11

N2 - Peroxynitrite (ONOO−), one reactive oxygen species, is related to various physiological and pathological activities. However, detailed mechanisms of peroxynitrite in biological systems are not thoroughly understood. Thus, the accurate detection of peroxynitrite with rapid response is essential for revealing its functions. Herein, we present a far red fluorescent probe Mito-WQPhOH to ratiometric monitor ONOO− in vitro and living cells. Mito-WQPhOH showed the merits of fast response (<20 s), outstanding selectivity and excellent sensitivity towards ONOO−. Importantly, owing to the good water-solubility, great biocompatibility and excellent mitochondria targeting of Mito-WQPhOH, it was used for ratiometric imaging endogenous ONOO− in living macrophages. Therefore, Mito-WQPhOH could act as a potential tool to reveal mechanisms of ONOO− in biological systems.

AB - Peroxynitrite (ONOO−), one reactive oxygen species, is related to various physiological and pathological activities. However, detailed mechanisms of peroxynitrite in biological systems are not thoroughly understood. Thus, the accurate detection of peroxynitrite with rapid response is essential for revealing its functions. Herein, we present a far red fluorescent probe Mito-WQPhOH to ratiometric monitor ONOO− in vitro and living cells. Mito-WQPhOH showed the merits of fast response (<20 s), outstanding selectivity and excellent sensitivity towards ONOO−. Importantly, owing to the good water-solubility, great biocompatibility and excellent mitochondria targeting of Mito-WQPhOH, it was used for ratiometric imaging endogenous ONOO− in living macrophages. Therefore, Mito-WQPhOH could act as a potential tool to reveal mechanisms of ONOO− in biological systems.

KW - Bioimaging

KW - Fluorescent probe

KW - Mitochondria

KW - Peroxynitrite

KW - Ratiometric determination

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U2 - 10.1016/j.dyepig.2019.107609

DO - 10.1016/j.dyepig.2019.107609

M3 - Article

AN - SCOPUS:85067054460

SN - 0143-7208

VL - 170

JO - Dyes and Pigments

JF - Dyes and Pigments

M1 - 107609

ER -

A mitochondria-targeted far red fluorescent probe for ratiometric imaging of endogenous peroxynitrite

Abstract

Keywords

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