A microfluidic cytometer with integrated on-chip optical systems for white blood cell analysis

Xinyue Su, Tao Peng, Qin Li, Zewen Wei*, Xuantao Su*

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

1 Citation (Scopus)

Abstract

White blood cells are a significant part of immune system, which can prevent human body from infection and invasion of foreign invaders. The count and recognition of white blood cells plays an important role in modern clinical practice. There is an urgent need to modify the conventional methods (such as cytometry), which are time-consuming and labor-intensive for white blood cell counting. This paper describes a microfluidic cytometer for white blood cells integrated a three-dimensional hydrodynamic focusing system and on-chip optical components, which can realize single-cell fluid flow and single-cell detection. Through the experiment, the device achieves the hydrodynamic focusing of cell flow and the detection of side scatter and fluorescence. For classifying and counting of white blood cells, we further perform an experiment using blood samples and get fairly good results.

Original languageEnglish
Title of host publicationOptics in Health Care and Biomedical Optics XI
EditorsQingming Luo, Xingde Li, Ying Gu, Dan Zhu
PublisherSPIE
ISBN (Electronic)9781510646490
DOIs
Publication statusPublished - 2021
EventOptics in Health Care and Biomedical Optics XI 2021 - Nantong, China
Duration: 10 Oct 202112 Oct 2021

Publication series

NameProceedings of SPIE - The International Society for Optical Engineering
Volume11900
ISSN (Print)0277-786X
ISSN (Electronic)1996-756X

Conference

ConferenceOptics in Health Care and Biomedical Optics XI 2021
Country/TerritoryChina
CityNantong
Period10/10/2112/10/21

Keywords

  • Microfluidic chip
  • On-chip Microlens
  • Three-dimensional hydrodynamic focusing
  • White blood cells counts

Fingerprint

Dive into the research topics of 'A microfluidic cytometer with integrated on-chip optical systems for white blood cell analysis'. Together they form a unique fingerprint.

Cite this