A highly selective fluorescent probe for monitoring exogenous and endogenous ONOO− fluctuations in HeLa cells

Wei Shu; Yulong Wu; Qingxia Duan; Shunping Zang; Sa Su; Jing Jing; Xiaoling Zhang

doi:10.1016/j.dyepig.2019.108069

A highly selective fluorescent probe for monitoring exogenous and endogenous ONOO⁻ fluctuations in HeLa cells

Wei Shu, Yulong Wu, Qingxia Duan, Shunping Zang, Sa Su, Jing Jing, Xiaoling Zhang^*

^*Corresponding author for this work

School of Chemistry and Chemical Engineering

Beijing Institute of Technology

Research output: Contribution to journal › Article › peer-review

25 Citations (Scopus)

Abstract

As a typical reactive oxygen species, peroxynitrite (ONOO⁻) plays vital roles in various biological functions and pharmacological activities. Specific, sensitive and rapid fluorescent probes are helpful tools for monitoring endogenous peroxynitrite. In this work, a highly selective fluorescent probe BTCBE was designed based on benzothiazole-2-acetonitrile and 4-formylphenylboronic acid pinacol cyclic ester. BTCBE showed the merits of rapid response (<1 min) and prominent sensitivity (LOD = 4.7 nM). It showed an excellent selectivity towards ONOO⁻ over NaClO and H₂O₂. Moreover, BTCBE was successfully used for bioimaging exogenous and endogenous ONOO⁻ in HeLa cells.

Original language	English
Article number	108069
Journal	Dyes and Pigments
Volume	175
DOIs	https://doi.org/10.1016/j.dyepig.2019.108069
Publication status	Published - Apr 2020

Keywords

Bioimaging
Colorimetric
Cyanovinylene dye
Fluorescent probe
Peroxynitrite

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10.1016/j.dyepig.2019.108069

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title = "A highly selective fluorescent probe for monitoring exogenous and endogenous ONOO− fluctuations in HeLa cells",

abstract = "As a typical reactive oxygen species, peroxynitrite (ONOO−) plays vital roles in various biological functions and pharmacological activities. Specific, sensitive and rapid fluorescent probes are helpful tools for monitoring endogenous peroxynitrite. In this work, a highly selective fluorescent probe BTCBE was designed based on benzothiazole-2-acetonitrile and 4-formylphenylboronic acid pinacol cyclic ester. BTCBE showed the merits of rapid response (<1 min) and prominent sensitivity (LOD = 4.7 nM). It showed an excellent selectivity towards ONOO− over NaClO and H2O2. Moreover, BTCBE was successfully used for bioimaging exogenous and endogenous ONOO− in HeLa cells.",

keywords = "Bioimaging, Colorimetric, Cyanovinylene dye, Fluorescent probe, Peroxynitrite",

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T1 - A highly selective fluorescent probe for monitoring exogenous and endogenous ONOO− fluctuations in HeLa cells

AU - Shu, Wei

AU - Wu, Yulong

AU - Duan, Qingxia

AU - Zang, Shunping

AU - Su, Sa

AU - Jing, Jing

AU - Zhang, Xiaoling

PY - 2020/4

Y1 - 2020/4

N2 - As a typical reactive oxygen species, peroxynitrite (ONOO−) plays vital roles in various biological functions and pharmacological activities. Specific, sensitive and rapid fluorescent probes are helpful tools for monitoring endogenous peroxynitrite. In this work, a highly selective fluorescent probe BTCBE was designed based on benzothiazole-2-acetonitrile and 4-formylphenylboronic acid pinacol cyclic ester. BTCBE showed the merits of rapid response (<1 min) and prominent sensitivity (LOD = 4.7 nM). It showed an excellent selectivity towards ONOO− over NaClO and H2O2. Moreover, BTCBE was successfully used for bioimaging exogenous and endogenous ONOO− in HeLa cells.

AB - As a typical reactive oxygen species, peroxynitrite (ONOO−) plays vital roles in various biological functions and pharmacological activities. Specific, sensitive and rapid fluorescent probes are helpful tools for monitoring endogenous peroxynitrite. In this work, a highly selective fluorescent probe BTCBE was designed based on benzothiazole-2-acetonitrile and 4-formylphenylboronic acid pinacol cyclic ester. BTCBE showed the merits of rapid response (<1 min) and prominent sensitivity (LOD = 4.7 nM). It showed an excellent selectivity towards ONOO− over NaClO and H2O2. Moreover, BTCBE was successfully used for bioimaging exogenous and endogenous ONOO− in HeLa cells.

KW - Bioimaging

KW - Colorimetric

KW - Cyanovinylene dye

KW - Fluorescent probe

KW - Peroxynitrite

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U2 - 10.1016/j.dyepig.2019.108069

DO - 10.1016/j.dyepig.2019.108069

M3 - Article

AN - SCOPUS:85076843210

SN - 0143-7208

VL - 175

JO - Dyes and Pigments

JF - Dyes and Pigments

M1 - 108069

ER -

A highly selective fluorescent probe for monitoring exogenous and endogenous ONOO⁻ fluctuations in HeLa cells

Abstract

Keywords

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