A colorimetric and ratiometric fluorescent probe for quantification of bovine serum albumin

Xiaodan Zeng, Xiaoling Zhang*, Baocun Zhu, Hongying Jia, Yamin Li, Juan Xue

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

30 Citations (Scopus)

Abstract

A 4-aminonaphthalimide-based ratiometric fluorescent probe 1 employing the internal charge transfer (ICT) mechanism was designed and synthesized to detect bovine serum albumin (BSA). The interaction of 1 and BSA was investigated by fluorescence and UV-vis absorption spectroscopy. Upon treatment with BSA, the probe successfully exhibited a ratiometric fluorescent response at 540 nm and 480 nm. The fluorescent intensity ratio at 540 nm and 480 nm (F 540/F480) increases linearly with BSA concentration in the range of 0-75.0 μg mL-1 and the detection limit was about 2.4 ng mL-1. Our strategy is expected to provide a methodology to quantify BSA either by a normal or by a ratiometric and colorimetric way with high sensitivity.

Original languageEnglish
Pages (from-to)4008-4012
Number of pages5
JournalThe Analyst
Volume136
Issue number19
DOIs
Publication statusPublished - 7 Oct 2011

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