A colorimetric and fluorescent chemodosimeter for discriminative and simultaneous quantification of cysteine and homocysteine

Liguo Wang; Qi Zhou; Baocun Zhu; Liangguo Yan; Zhenmin Ma; Bin Du; Xiaoling Zhang

doi:10.1016/j.dyepig.2012.05.006

A colorimetric and fluorescent chemodosimeter for discriminative and simultaneous quantification of cysteine and homocysteine

Liguo Wang, Qi Zhou, Baocun Zhu^*, Liangguo Yan, Zhenmin Ma, Bin Du, Xiaoling Zhang

^*Corresponding author for this work

School of Chemistry and Chemical Engineering

University of Jinan

Research output: Contribution to journal › Article › peer-review

57 Citations (Scopus)

Abstract

A highly selective, visible-light-excited and OFF-ON fluorescent chemodosimeter 1 employed conjugate addition/cyclization sequence mechanism, was designed and synthesized to discriminatively detect cysteine (Cys) and homocysteine (Hcy). The addition of Cys and Hcy resulted in the color of the solution of 1 changing from colorless to green under the simulation of physiological condition, and 1 could serve as a "naked-eye" indicator. Our chemodosimeter can detect Cys and Hcy quantitatively by fluorescence spectrometry method with a detection limit of 0.5 μM (for Cys) and 0.8 μM (for Hcy). To the best of our knowledge, 1 is the first visual and visible-light-excited fluorescent indicator for discriminative and simultaneous detection of Cys and Hcy. Furthermore, the mechanism of the reaction between 1 and Cys was confirmed using ESI-MS and fluorescence spectra.

Original language	English
Pages (from-to)	275-279
Number of pages	5
Journal	Dyes and Pigments
Volume	95
Issue number	2
DOIs	https://doi.org/10.1016/j.dyepig.2012.05.006
Publication status	Published - Nov 2012

Keywords

Colorimetric
Cysteine
Fluorescein
Fluorescent chemodosimeter
Homocysteine
Simultaneous detection

Access to Document

10.1016/j.dyepig.2012.05.006

Cite this

@article{d8e9e7bfcd7e4f5aa3141c6c88e39035,

title = "A colorimetric and fluorescent chemodosimeter for discriminative and simultaneous quantification of cysteine and homocysteine",

abstract = "A highly selective, visible-light-excited and OFF-ON fluorescent chemodosimeter 1 employed conjugate addition/cyclization sequence mechanism, was designed and synthesized to discriminatively detect cysteine (Cys) and homocysteine (Hcy). The addition of Cys and Hcy resulted in the color of the solution of 1 changing from colorless to green under the simulation of physiological condition, and 1 could serve as a {"}naked-eye{"} indicator. Our chemodosimeter can detect Cys and Hcy quantitatively by fluorescence spectrometry method with a detection limit of 0.5 μM (for Cys) and 0.8 μM (for Hcy). To the best of our knowledge, 1 is the first visual and visible-light-excited fluorescent indicator for discriminative and simultaneous detection of Cys and Hcy. Furthermore, the mechanism of the reaction between 1 and Cys was confirmed using ESI-MS and fluorescence spectra.",

keywords = "Colorimetric, Cysteine, Fluorescein, Fluorescent chemodosimeter, Homocysteine, Simultaneous detection",

author = "Liguo Wang and Qi Zhou and Baocun Zhu and Liangguo Yan and Zhenmin Ma and Bin Du and Xiaoling Zhang",

year = "2012",

month = nov,

doi = "10.1016/j.dyepig.2012.05.006",

language = "English",

volume = "95",

pages = "275--279",

journal = "Dyes and Pigments",

issn = "0143-7208",

publisher = "Elsevier B.V.",

number = "2",

}

TY - JOUR

T1 - A colorimetric and fluorescent chemodosimeter for discriminative and simultaneous quantification of cysteine and homocysteine

AU - Wang, Liguo

AU - Zhou, Qi

AU - Zhu, Baocun

AU - Yan, Liangguo

AU - Ma, Zhenmin

AU - Du, Bin

AU - Zhang, Xiaoling

PY - 2012/11

Y1 - 2012/11

N2 - A highly selective, visible-light-excited and OFF-ON fluorescent chemodosimeter 1 employed conjugate addition/cyclization sequence mechanism, was designed and synthesized to discriminatively detect cysteine (Cys) and homocysteine (Hcy). The addition of Cys and Hcy resulted in the color of the solution of 1 changing from colorless to green under the simulation of physiological condition, and 1 could serve as a "naked-eye" indicator. Our chemodosimeter can detect Cys and Hcy quantitatively by fluorescence spectrometry method with a detection limit of 0.5 μM (for Cys) and 0.8 μM (for Hcy). To the best of our knowledge, 1 is the first visual and visible-light-excited fluorescent indicator for discriminative and simultaneous detection of Cys and Hcy. Furthermore, the mechanism of the reaction between 1 and Cys was confirmed using ESI-MS and fluorescence spectra.

AB - A highly selective, visible-light-excited and OFF-ON fluorescent chemodosimeter 1 employed conjugate addition/cyclization sequence mechanism, was designed and synthesized to discriminatively detect cysteine (Cys) and homocysteine (Hcy). The addition of Cys and Hcy resulted in the color of the solution of 1 changing from colorless to green under the simulation of physiological condition, and 1 could serve as a "naked-eye" indicator. Our chemodosimeter can detect Cys and Hcy quantitatively by fluorescence spectrometry method with a detection limit of 0.5 μM (for Cys) and 0.8 μM (for Hcy). To the best of our knowledge, 1 is the first visual and visible-light-excited fluorescent indicator for discriminative and simultaneous detection of Cys and Hcy. Furthermore, the mechanism of the reaction between 1 and Cys was confirmed using ESI-MS and fluorescence spectra.

KW - Colorimetric

KW - Cysteine

KW - Fluorescein

KW - Fluorescent chemodosimeter

KW - Homocysteine

KW - Simultaneous detection

UR - http://www.scopus.com/inward/record.url?scp=84862278265&partnerID=8YFLogxK

U2 - 10.1016/j.dyepig.2012.05.006

DO - 10.1016/j.dyepig.2012.05.006

M3 - Article

AN - SCOPUS:84862278265

SN - 0143-7208

VL - 95

SP - 275

EP - 279

JO - Dyes and Pigments

JF - Dyes and Pigments

IS - 2

ER -

A colorimetric and fluorescent chemodosimeter for discriminative and simultaneous quantification of cysteine and homocysteine

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Cite this