1-acetyl-6, 7-dihydroxyl-1, 2, 3, 4-tetrahydroisoquinoline (ADTIQ) induced apoptosis and decreased the expression of dopamine transporter in SH-SY5Y cells

Bing Jie Xie; Yu Lin Deng

doi:10.15918/j.tbit1001-0645.2015.supplement1.028

1-acetyl-6, 7-dihydroxyl-1, 2, 3, 4-tetrahydroisoquinoline (ADTIQ) induced apoptosis and decreased the expression of dopamine transporter in SH-SY5Y cells

Bing Jie Xie, Yu Lin Deng

School of Medical and Technology

Beijing Institute of Technology

Research output: Contribution to journal › Article › peer-review

Abstract

1-acetyl-6, 7-dihydroxyl-1, 2, 3, 4-Tetrahydroisoquinoline(ADTIQ) is a novel endogenous neurotoxins, and related to glucose metabolism. Therefore, ADTIQ may play a key role in increasing the risk for PD in patients with diabetes. But the neurotoxicity of ADTIQ is still unclear. Here, we try to investigate the neurotoxicity of ADTIQ on SH-SY5Y cells. The Annexin V-FITC/PI, MTT, Caspase 3/7 analyses were used to measure the cell viability and apoptosis of ADTIQ, and dopamine transporter (DAT) levels were detected by western blot. The results show that ADTIQ reduced the cell viability and induces apoptosis in SH-SY5Y cells, it also decreases the expression of DAT, suggesting that ADTIQ involved in the pathogenesis of PD. Diabetes induced the increase of ADTIQ, which could be related with Hyperglycemia and death of dopaminergic neurons.

Original language	English
Pages (from-to)	128-131 and 141
Journal	Beijing Ligong Daxue Xuebao/Transaction of Beijing Institute of Technology
Volume	35
DOIs	https://doi.org/10.15918/j.tbit1001-0645.2015.supplement1.028
Publication status	Published - 1 Jul 2015

Keywords

1-acetyl-6, 7-dihydroxyl-1, 2, 3, 4-tetrahydroisoquinoline (ADTIQ)
Diabetes
Dopamine transporter (DAT)
Parkinson's disease (PD)

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.15918/j.tbit1001-0645.2015.supplement1.028

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Xie, B. J., & Deng, Y. L. (2015). 1-acetyl-6, 7-dihydroxyl-1, 2, 3, 4-tetrahydroisoquinoline (ADTIQ) induced apoptosis and decreased the expression of dopamine transporter in SH-SY5Y cells. Beijing Ligong Daxue Xuebao/Transaction of Beijing Institute of Technology, 35, 128-131 and 141. https://doi.org/10.15918/j.tbit1001-0645.2015.supplement1.028

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title = "1-acetyl-6, 7-dihydroxyl-1, 2, 3, 4-tetrahydroisoquinoline (ADTIQ) induced apoptosis and decreased the expression of dopamine transporter in SH-SY5Y cells",

abstract = "1-acetyl-6, 7-dihydroxyl-1, 2, 3, 4-Tetrahydroisoquinoline(ADTIQ) is a novel endogenous neurotoxins, and related to glucose metabolism. Therefore, ADTIQ may play a key role in increasing the risk for PD in patients with diabetes. But the neurotoxicity of ADTIQ is still unclear. Here, we try to investigate the neurotoxicity of ADTIQ on SH-SY5Y cells. The Annexin V-FITC/PI, MTT, Caspase 3/7 analyses were used to measure the cell viability and apoptosis of ADTIQ, and dopamine transporter (DAT) levels were detected by western blot. The results show that ADTIQ reduced the cell viability and induces apoptosis in SH-SY5Y cells, it also decreases the expression of DAT, suggesting that ADTIQ involved in the pathogenesis of PD. Diabetes induced the increase of ADTIQ, which could be related with Hyperglycemia and death of dopaminergic neurons.",

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1-acetyl-6, 7-dihydroxyl-1, 2, 3, 4-tetrahydroisoquinoline (ADTIQ) induced apoptosis and decreased the expression of dopamine transporter in SH-SY5Y cells. / Xie, Bing Jie; Deng, Yu Lin.
In: Beijing Ligong Daxue Xuebao/Transaction of Beijing Institute of Technology, Vol. 35, 01.07.2015, p. 128-131 and 141.

Research output: Contribution to journal › Article › peer-review

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AU - Deng, Yu Lin

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AB - 1-acetyl-6, 7-dihydroxyl-1, 2, 3, 4-Tetrahydroisoquinoline(ADTIQ) is a novel endogenous neurotoxins, and related to glucose metabolism. Therefore, ADTIQ may play a key role in increasing the risk for PD in patients with diabetes. But the neurotoxicity of ADTIQ is still unclear. Here, we try to investigate the neurotoxicity of ADTIQ on SH-SY5Y cells. The Annexin V-FITC/PI, MTT, Caspase 3/7 analyses were used to measure the cell viability and apoptosis of ADTIQ, and dopamine transporter (DAT) levels were detected by western blot. The results show that ADTIQ reduced the cell viability and induces apoptosis in SH-SY5Y cells, it also decreases the expression of DAT, suggesting that ADTIQ involved in the pathogenesis of PD. Diabetes induced the increase of ADTIQ, which could be related with Hyperglycemia and death of dopaminergic neurons.

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KW - Dopamine transporter (DAT)

KW - Parkinson's disease (PD)

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1-acetyl-6, 7-dihydroxyl-1, 2, 3, 4-tetrahydroisoquinoline (ADTIQ) induced apoptosis and decreased the expression of dopamine transporter in SH-SY5Y cells

Abstract

Keywords

UN SDGs

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