基于单步驱动的激光共焦显微镜快速定焦方法

Aiming at the problem of slow speed and poor precision of focusing in existing confocal microscopes, this paper proposes a fast focusing method based on single-step driving laser confocal microscope. The method firstly acquires data based on single-step driving of axial scanner. Then, by fitting the interval-optimized data processing method, the position of the focus identification can be quickly and accurately obtained, thereby improving the imaging speed and accuracy of the laser confocal microscope. Theoretical analysis and experimental results show that compared with the existing focus identification method, the method increases the focus identification speed by 5.64 times in the coarse focus identification stage and 3.08 times in the quasi-determined focus stage, which effectively improves the axial focus identification speed and speed of the confocal microscope.