Abstract
Yeast has been well used as a typical microbial platform to make fermented fine chemicals. However, various stress conditions and abnormal metabolic milieu severely restrict the production costs and benefits. One effective way to resolve such bottlenecks is to engineer transcription factors (TFs) to enhance strain tolerance and production efficiency through remodeling the transcript levels of different stress resistant genes. The value of TFs engineering is reviewed from two aspects: novel strategies for the enhancement of both tolerance and yield by TFs engineering are examined. In addition, the applications of artificial transcription factors (ATFs)based fabricating in metabolic fluxes optimization and quantitative evaluation are discussed. Lastly, we discuss challenges and potential solutions in exploiting TFs engineering for bio-based economic products.
| Translated title of the contribution | Transcription Factor Engineering Harnesses Metabolic Networks to Meet Efficient Production in Cell Factories |
|---|---|
| Original language | Chinese (Traditional) |
| Pages (from-to) | 55-63 |
| Number of pages | 9 |
| Journal | China Biotechnology |
| Volume | 41 |
| Issue number | 4 |
| DOIs | |
| Publication status | Published - 2021 |