UV-visible and fluorescence spectra of 5,10,15,20-tetra(4-hydroxypheny)porphyrin in triton X-100 micelle

The interactions between a fluorescent molecule, 5,10,15,20-tetra(4-hydroxyphenyl)porphyrin P₀, and Triton X-100 micelle are investigated by UV-vis absorption and fluorescence spectra. The results show that the deprotonation of P₀ has occurred in micelk solutions in the basic titration process. By means of analyzing the relationship between the solubilizing location of the porphyrin in TX-100 micelle and the microenvironment polarity, it is shown that P₀ is involved in a transfer process from the inner to the outer surface of TX-100 micelle as the hydrophilic ability is increased. At the same time, the reasons of fluorescence quenching effect are explained primarily.