The optimization of proteins assay under extreme acidic condition with capillary electrophoresis

Li Qian; Qu Feng; Hongmei; Gong; Deng Yulin

doi:10.1109/ICCME.2007.4382037

The optimization of proteins assay under extreme acidic condition with capillary electrophoresis

Li Qian, Qu Feng^*, Hongmei, Gong, Deng Yulin

^*此作品的通讯作者

医学技术学院

Beijing Institute of Technology

科研成果: 书/报告/会议事项章节 › 会议稿件 › 同行评审

摘要

Capillary electrophoresis (CE) was an advanced method for protein separation. Under extreme acidic condition(pH <3), fused silica capillary inner wall was protonated, so that the electrostatic adsorption between protein and inner wall of capillary could be suppressed effectively, which caused the resolution and column efficiency were improved. In this paper, four model proteins were separated under extreme acidic condition, and the separation rules were investigated by optimizing conditions, such as changing pH value and ion-strength of running buffer, adding high molecular polymer methylcellulose (MC) into running buffer, adding organic solvent methanol into running buffer.

源语言	英语
主期刊名	2007 IEEE/ICME International Conference on Complex Medical Engineering, CME 2007
页	1698-1701
页数	4
DOI	https://doi.org/10.1109/ICCME.2007.4382037
出版状态	已出版 - 2007
活动	2007 IEEE/ICME International Conference on Complex Medical Engineering, CME 2007 - Beijing, 中国期限: 23 5月 2007 → 27 5月 2007

出版系列

姓名	2007 IEEE/ICME International Conference on Complex Medical Engineering, CME 2007

会议

会议	2007 IEEE/ICME International Conference on Complex Medical Engineering, CME 2007
国家/地区	中国
市	Beijing
时期	23/05/07 → 27/05/07

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10.1109/ICCME.2007.4382037

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Qian, L., Feng, Q., Hongmei, Gong, & Yulin, D. (2007). The optimization of proteins assay under extreme acidic condition with capillary electrophoresis. 在 2007 IEEE/ICME International Conference on Complex Medical Engineering, CME 2007 (页码 1698-1701). 文章 4382037 (2007 IEEE/ICME International Conference on Complex Medical Engineering, CME 2007). https://doi.org/10.1109/ICCME.2007.4382037

@inproceedings{c9eb648281b54389ad389bd9f6a31fea,

title = "The optimization of proteins assay under extreme acidic condition with capillary electrophoresis",

abstract = "Capillary electrophoresis (CE) was an advanced method for protein separation. Under extreme acidic condition(pH <3), fused silica capillary inner wall was protonated, so that the electrostatic adsorption between protein and inner wall of capillary could be suppressed effectively, which caused the resolution and column efficiency were improved. In this paper, four model proteins were separated under extreme acidic condition, and the separation rules were investigated by optimizing conditions, such as changing pH value and ion-strength of running buffer, adding high molecular polymer methylcellulose (MC) into running buffer, adding organic solvent methanol into running buffer.",

author = "Li Qian and Qu Feng and Hongmei and Gong and Deng Yulin",

year = "2007",

doi = "10.1109/ICCME.2007.4382037",

language = "English",

isbn = "1424410789",

series = "2007 IEEE/ICME International Conference on Complex Medical Engineering, CME 2007",

pages = "1698--1701",

booktitle = "2007 IEEE/ICME International Conference on Complex Medical Engineering, CME 2007",

note = "2007 IEEE/ICME International Conference on Complex Medical Engineering, CME 2007 ; Conference date: 23-05-2007 Through 27-05-2007",

}

Qian, L, Feng, Q, Hongmei, Gong, & Yulin, D 2007, The optimization of proteins assay under extreme acidic condition with capillary electrophoresis. 在 2007 IEEE/ICME International Conference on Complex Medical Engineering, CME 2007., 4382037, 2007 IEEE/ICME International Conference on Complex Medical Engineering, CME 2007, 页码 1698-1701, 2007 IEEE/ICME International Conference on Complex Medical Engineering, CME 2007, Beijing, 中国, 23/05/07. https://doi.org/10.1109/ICCME.2007.4382037

The optimization of proteins assay under extreme acidic condition with capillary electrophoresis. / Qian, Li; Feng, Qu; Hongmei 等.
2007 IEEE/ICME International Conference on Complex Medical Engineering, CME 2007. 2007. 页码 1698-1701 4382037 (2007 IEEE/ICME International Conference on Complex Medical Engineering, CME 2007).

科研成果: 书/报告/会议事项章节 › 会议稿件 › 同行评审

TY - GEN

T1 - The optimization of proteins assay under extreme acidic condition with capillary electrophoresis

AU - Qian, Li

AU - Feng, Qu

AU - Hongmei,

AU - Gong,

AU - Yulin, Deng

PY - 2007

Y1 - 2007

N2 - Capillary electrophoresis (CE) was an advanced method for protein separation. Under extreme acidic condition(pH <3), fused silica capillary inner wall was protonated, so that the electrostatic adsorption between protein and inner wall of capillary could be suppressed effectively, which caused the resolution and column efficiency were improved. In this paper, four model proteins were separated under extreme acidic condition, and the separation rules were investigated by optimizing conditions, such as changing pH value and ion-strength of running buffer, adding high molecular polymer methylcellulose (MC) into running buffer, adding organic solvent methanol into running buffer.

AB - Capillary electrophoresis (CE) was an advanced method for protein separation. Under extreme acidic condition(pH <3), fused silica capillary inner wall was protonated, so that the electrostatic adsorption between protein and inner wall of capillary could be suppressed effectively, which caused the resolution and column efficiency were improved. In this paper, four model proteins were separated under extreme acidic condition, and the separation rules were investigated by optimizing conditions, such as changing pH value and ion-strength of running buffer, adding high molecular polymer methylcellulose (MC) into running buffer, adding organic solvent methanol into running buffer.

UR - http://www.scopus.com/inward/record.url?scp=48149115329&partnerID=8YFLogxK

U2 - 10.1109/ICCME.2007.4382037

DO - 10.1109/ICCME.2007.4382037

M3 - Conference contribution

AN - SCOPUS:48149115329

SN - 1424410789

SN - 9781424410781

T3 - 2007 IEEE/ICME International Conference on Complex Medical Engineering, CME 2007

SP - 1698

EP - 1701

BT - 2007 IEEE/ICME International Conference on Complex Medical Engineering, CME 2007

T2 - 2007 IEEE/ICME International Conference on Complex Medical Engineering, CME 2007

Y2 - 23 May 2007 through 27 May 2007

ER -

Qian L, Feng Q, Hongmei, Gong , Yulin D. The optimization of proteins assay under extreme acidic condition with capillary electrophoresis. 在 2007 IEEE/ICME International Conference on Complex Medical Engineering, CME 2007. 2007. 页码 1698-1701. 4382037. (2007 IEEE/ICME International Conference on Complex Medical Engineering, CME 2007). doi: 10.1109/ICCME.2007.4382037

The optimization of proteins assay under extreme acidic condition with capillary electrophoresis

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