The method of immobilized pH gradient of CIEF used in protein separation

Yao Xie, Lina Geng, Feng Qu, Aiqin Luo, Yulin Deng*

*此作品的通讯作者

科研成果: 期刊稿件文章同行评审

1 引用 (Scopus)

摘要

An immobilized pH gradient method had been developed for proteins separation by two-step capillary isoelectric focusing based on chemical bonding. The double functional reagent, N,N′-carbonyldiimidazole was introduced in the capillary as a linkage in the immobilization reactions. Moreover, the commercial ampholytes (CAs) were distributed according to their pI values in the condition of electric field, and then connected with N,N′- carbonyldiimidazole by chemical bonding to realize the immobilized pH gradient in capillary. A test sample containing a protein mixture of hemoglobin (Hb), bovine serum albumin (BSA) and pepsin was separated by using this method.

源语言英语
页(从-至)461-464
页数4
期刊Chemistry Bulletin / Huaxue Tongbao
71
6
出版状态已出版 - 2008

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