Tenebrionibacter intestinalis gen. nov., sp. nov., a member of a novel genus of the family Enterobacteriaceae, isolated from the gut of the plastic-eating mealworm Tenebrio molitor L.

A Gram-negative, white-pigmented, motile and rod-shaped strain, BIT-L3^T, was isolated from the gut of plastic-eating mealworm Tenebrio molitor L. Its taxonomic position was determined by using a polyphasic approach. A preliminary analysis based on the 16S rRNA gene sequence (1445 bp) revealed that this strain was closely related to the members within the family Enterobacteriaceae. Phylogenetic trees based on the concatenated partial sequences of seven housekeeping genes (atpD, gyrB, infB, rpoB, pyrG, fusA, leuS) and genome sequences further showed that strain BIT-L3^T constituted a separate lineage within the family Enterobacteriaceae. In silico DNA–DNA hybridization values and average nucleotide identity values between strain BIT-L3^T and its closest related species within the family Enterobacteriaceae were less than 21.8 and 76.7%, respectively. The major fatty acids (>5%) of strain BIT-L3^T were C_16:0, C_14:0, C_17:0 cyclo, summed feature 8 (comprising C_18:1 ω7c and/or C_18:1 ω6c), summed feature 3 (comprising C_16:1 ω7c and/or C_16:1 ω6c and/or iso-C_15:0 2-OH) and summed feature 2 (comprising iso-C_16:1 I/ C_14:0 3-OH and/or C_12:0 aldehyde and/or an unknown fatty acid of equivalent chain length 10.9525). Its genomic DNA G+C content was 53.7 mol%. Based on the results of phylogenetic, physiological and biochemical analyses, strain BIT-L3^T is considered to represent a novel species of a novel genus within the family Enterobacteriaceae, for which the name Tenebrionibacter intestinalis gen. nov., sp. nov. is proposed. The type strain is BIT-L3^T (=CCTCC AB 2020371^T=LMG 32222^T=TBRC 14825^T).