TY - GEN
T1 - Purification and identification of soluble Catechol-O-methyltransferase from rat liver
AU - Yang, Lina
AU - Yan, Jianhui
AU - Deng, Yulin
PY - 2010
Y1 - 2010
N2 - Soluble catechol-O-methytransferase (S-COMT) is an important target protein due to its involvement in some human disorders. The main scope of the work was to develop an approach to purify and identify S-COMT. Its isoelectric point was S.O determined with Offgel. A simplified process including extraction, selective precipitation, ammonium sulfate precipitation, gel nitration chromatography and anion exchange chromatography was established to obtain S-COMT of a certain purity degree, The molecular was 2SkD. Mass spectrometry and mascot were employed for further identification.
AB - Soluble catechol-O-methytransferase (S-COMT) is an important target protein due to its involvement in some human disorders. The main scope of the work was to develop an approach to purify and identify S-COMT. Its isoelectric point was S.O determined with Offgel. A simplified process including extraction, selective precipitation, ammonium sulfate precipitation, gel nitration chromatography and anion exchange chromatography was established to obtain S-COMT of a certain purity degree, The molecular was 2SkD. Mass spectrometry and mascot were employed for further identification.
KW - Identification
KW - Mass spectrometry
KW - Offgel isoelectric focusing
KW - Purification
KW - Solublecatechol-O-methyltransferase
UR - http://www.scopus.com/inward/record.url?scp=77956150907&partnerID=8YFLogxK
U2 - 10.1109/ICBBE.2010.5517422
DO - 10.1109/ICBBE.2010.5517422
M3 - Conference contribution
AN - SCOPUS:77956150907
SN - 9781424447138
T3 - 2010 4th International Conference on Bioinformatics and Biomedical Engineering, iCBBE 2010
BT - 2010 4th International Conference on Bioinformatics and Biomedical Engineering, iCBBE 2010
T2 - 4th International Conference on Bioinformatics and Biomedical Engineering, iCBBE 2010
Y2 - 18 June 2010 through 20 June 2010
ER -