Preliminary screening of peptide biomarker for early diagnosis of diabetes

Mei Zhang; Ke Ming Shan; Yu Lin Deng

doi:10.15918/j.tbit1001-0645.2015.supplement1.026

Preliminary screening of peptide biomarker for early diagnosis of diabetes

Mei Zhang^*, Ke Ming Shan, Yu Lin Deng

^*此作品的通讯作者

医学技术学院

科研成果: 期刊稿件 › 文章 › 同行评审

摘要

An in vitro glycation model was set up by glucose and human serum albumin (HSA) based on Maillard Reaction. HPLC-ESI TOF MS and ¹⁸O-labeling technique were employed to quantify glycation level of peptides cleaved from HSA. In order to screen glucose-sensitive peptides for being diabetic biomarker-candidates, the in vitro glycation model was set up and confirmed, and eleven biomarker-candidates were screened based on the model by HPLC-ESI TOF MS quantification. Eleven glucose-sensitive peptides were screened for being biomarker-candidates, which needs to be verified in clinical trials in the future.

源语言	英语
页（从-至）	119-122
页数	4
期刊	Beijing Ligong Daxue Xuebao/Transaction of Beijing Institute of Technology
卷	35
DOI	https://doi.org/10.15918/j.tbit1001-0645.2015.supplement1.026
出版状态	已出版 - 1 7月 2015

联合国可持续发展目标

此成果有助于实现下列可持续发展目标：

访问文件

10.15918/j.tbit1001-0645.2015.supplement1.026

其它文件与链接

链接到 Scopus 的出版物

引用此

@article{50ff150928004b9ab17745e39c398b0f,

title = "Preliminary screening of peptide biomarker for early diagnosis of diabetes",

abstract = "An in vitro glycation model was set up by glucose and human serum albumin (HSA) based on Maillard Reaction. HPLC-ESI TOF MS and 18O-labeling technique were employed to quantify glycation level of peptides cleaved from HSA. In order to screen glucose-sensitive peptides for being diabetic biomarker-candidates, the in vitro glycation model was set up and confirmed, and eleven biomarker-candidates were screened based on the model by HPLC-ESI TOF MS quantification. Eleven glucose-sensitive peptides were screened for being biomarker-candidates, which needs to be verified in clinical trials in the future.",

keywords = "Biomarker, Diabetes, HPLC-ESI TOF MS, Human serum albumin, O-labeling",

author = "Mei Zhang and Shan, {Ke Ming} and Deng, {Yu Lin}",

year = "2015",

month = jul,

day = "1",

doi = "10.15918/j.tbit1001-0645.2015.supplement1.026",

language = "English",

volume = "35",

pages = "119--122",

journal = "Beijing Ligong Daxue Xuebao/Transaction of Beijing Institute of Technology",

issn = "1001-0645",

publisher = "Beijing Institute of Technology",

}

TY - JOUR

T1 - Preliminary screening of peptide biomarker for early diagnosis of diabetes

AU - Zhang, Mei

AU - Shan, Ke Ming

AU - Deng, Yu Lin

PY - 2015/7/1

Y1 - 2015/7/1

N2 - An in vitro glycation model was set up by glucose and human serum albumin (HSA) based on Maillard Reaction. HPLC-ESI TOF MS and 18O-labeling technique were employed to quantify glycation level of peptides cleaved from HSA. In order to screen glucose-sensitive peptides for being diabetic biomarker-candidates, the in vitro glycation model was set up and confirmed, and eleven biomarker-candidates were screened based on the model by HPLC-ESI TOF MS quantification. Eleven glucose-sensitive peptides were screened for being biomarker-candidates, which needs to be verified in clinical trials in the future.

AB - An in vitro glycation model was set up by glucose and human serum albumin (HSA) based on Maillard Reaction. HPLC-ESI TOF MS and 18O-labeling technique were employed to quantify glycation level of peptides cleaved from HSA. In order to screen glucose-sensitive peptides for being diabetic biomarker-candidates, the in vitro glycation model was set up and confirmed, and eleven biomarker-candidates were screened based on the model by HPLC-ESI TOF MS quantification. Eleven glucose-sensitive peptides were screened for being biomarker-candidates, which needs to be verified in clinical trials in the future.

KW - Biomarker

KW - Diabetes

KW - HPLC-ESI TOF MS

KW - Human serum albumin

KW - O-labeling

UR - http://www.scopus.com/inward/record.url?scp=84943424135&partnerID=8YFLogxK

U2 - 10.15918/j.tbit1001-0645.2015.supplement1.026

DO - 10.15918/j.tbit1001-0645.2015.supplement1.026

M3 - Article

AN - SCOPUS:84943424135

SN - 1001-0645

VL - 35

SP - 119

EP - 122

JO - Beijing Ligong Daxue Xuebao/Transaction of Beijing Institute of Technology

JF - Beijing Ligong Daxue Xuebao/Transaction of Beijing Institute of Technology

ER -

Preliminary screening of peptide biomarker for early diagnosis of diabetes

摘要

联合国可持续发展目标

访问文件

其它文件与链接

指纹

引用此