Hierarchical and ultra-sensitive amyloid beta oligomer sensor for practical applications

Jieling Qin; Soochan Kim; Misuk Cho; Youngkwan Lee

doi:10.1016/j.cej.2020.126055

Hierarchical and ultra-sensitive amyloid beta oligomer sensor for practical applications

Jieling Qin, Soochan Kim, Misuk Cho^*, Youngkwan Lee

^*此作品的通讯作者

科研成果: 期刊稿件 › 文章 › 同行评审

20 引用（Scopus）

摘要

In this work, an ultra-sensitive amyloid beta oligomer (AβO) sensor is presented in the detection range at atomolar level. A hierarchical gold dendrite (AuD) was electrochemically prepared on a gold substrate. Poly(pyrrole-3-carboxylic acid) [PPy-3-COOH] was electrodeposited on AuD to maintain the outstanding electrochemical properties, such as high conductivity and large surface area. After immobilization of the cellular prion protein (PrP^C) bioreceptor through amide bond formation, the electrochemical sensor was incubated in gradient concentrations of AβO. The impedance response of the dendritic PPy-3-COOH/PrP^C biosensor showed a proportional relationship to the increase of AβO concentration from 10⁻⁹ to 10 nM. The sensitivity at an atomolar level and high selectivity to AβO can be used in the diseased mice and blood test for the early diagnosis of Alzheimer's disease.

源语言	英语
文章编号	126055
期刊	Chemical Engineering Journal
卷	401
DOI	https://doi.org/10.1016/j.cej.2020.126055
出版状态	已出版 - 1 12月 2020
已对外发布	是

访问文件

10.1016/j.cej.2020.126055

其它文件与链接

链接到 Scopus 的出版物

引用此

@article{f397ea41ff0c4943a99eeea89567167e,

title = "Hierarchical and ultra-sensitive amyloid beta oligomer sensor for practical applications",

abstract = "In this work, an ultra-sensitive amyloid beta oligomer (AβO) sensor is presented in the detection range at atomolar level. A hierarchical gold dendrite (AuD) was electrochemically prepared on a gold substrate. Poly(pyrrole-3-carboxylic acid) [PPy-3-COOH] was electrodeposited on AuD to maintain the outstanding electrochemical properties, such as high conductivity and large surface area. After immobilization of the cellular prion protein (PrPC) bioreceptor through amide bond formation, the electrochemical sensor was incubated in gradient concentrations of AβO. The impedance response of the dendritic PPy-3-COOH/PrPC biosensor showed a proportional relationship to the increase of AβO concentration from 10−9 to 10 nM. The sensitivity at an atomolar level and high selectivity to AβO can be used in the diseased mice and blood test for the early diagnosis of Alzheimer's disease.",

keywords = "Amyloid beta oligomer, Functional conductive polymer, Hierarchical and dendritic structure, Impedance sensor",

author = "Jieling Qin and Soochan Kim and Misuk Cho and Youngkwan Lee",

note = "Publisher Copyright: {\textcopyright} 2020 Elsevier B.V.",

year = "2020",

month = dec,

day = "1",

doi = "10.1016/j.cej.2020.126055",

language = "English",

volume = "401",

journal = "Chemical Engineering Journal",

issn = "1385-8947",

publisher = "Elsevier B.V.",

}

TY - JOUR

T1 - Hierarchical and ultra-sensitive amyloid beta oligomer sensor for practical applications

AU - Qin, Jieling

AU - Kim, Soochan

AU - Cho, Misuk

AU - Lee, Youngkwan

PY - 2020/12/1

Y1 - 2020/12/1

N2 - In this work, an ultra-sensitive amyloid beta oligomer (AβO) sensor is presented in the detection range at atomolar level. A hierarchical gold dendrite (AuD) was electrochemically prepared on a gold substrate. Poly(pyrrole-3-carboxylic acid) [PPy-3-COOH] was electrodeposited on AuD to maintain the outstanding electrochemical properties, such as high conductivity and large surface area. After immobilization of the cellular prion protein (PrPC) bioreceptor through amide bond formation, the electrochemical sensor was incubated in gradient concentrations of AβO. The impedance response of the dendritic PPy-3-COOH/PrPC biosensor showed a proportional relationship to the increase of AβO concentration from 10−9 to 10 nM. The sensitivity at an atomolar level and high selectivity to AβO can be used in the diseased mice and blood test for the early diagnosis of Alzheimer's disease.

AB - In this work, an ultra-sensitive amyloid beta oligomer (AβO) sensor is presented in the detection range at atomolar level. A hierarchical gold dendrite (AuD) was electrochemically prepared on a gold substrate. Poly(pyrrole-3-carboxylic acid) [PPy-3-COOH] was electrodeposited on AuD to maintain the outstanding electrochemical properties, such as high conductivity and large surface area. After immobilization of the cellular prion protein (PrPC) bioreceptor through amide bond formation, the electrochemical sensor was incubated in gradient concentrations of AβO. The impedance response of the dendritic PPy-3-COOH/PrPC biosensor showed a proportional relationship to the increase of AβO concentration from 10−9 to 10 nM. The sensitivity at an atomolar level and high selectivity to AβO can be used in the diseased mice and blood test for the early diagnosis of Alzheimer's disease.

KW - Amyloid beta oligomer

KW - Functional conductive polymer

KW - Hierarchical and dendritic structure

KW - Impedance sensor

UR - http://www.scopus.com/inward/record.url?scp=85087503507&partnerID=8YFLogxK

U2 - 10.1016/j.cej.2020.126055

DO - 10.1016/j.cej.2020.126055

M3 - Article

AN - SCOPUS:85087503507

SN - 1385-8947

VL - 401

JO - Chemical Engineering Journal

JF - Chemical Engineering Journal

M1 - 126055

ER -

Hierarchical and ultra-sensitive amyloid beta oligomer sensor for practical applications

摘要

访问文件

其它文件与链接

指纹

引用此