TY - GEN
T1 - Generation of rotational flow in microfluidics device for formation of spheroid
AU - Kojima, Masaru
AU - Horade, Mitsuhiro
AU - Takai, Hirochika
AU - Ohara, Kenichi
AU - Tanikawa, Tamio
AU - Kamiyama, Kazuto
AU - Mae, Yasushi
AU - Arai, Tatsuo
N1 - Publisher Copyright:
© 2014 IEEE.
PY - 2015/1/9
Y1 - 2015/1/9
N2 - Spheroid is formed by making it agitate and circle in cell suspension. However, the injection of the cells used as pieces of spheroids, the recovery at the time of tissue formation, exchange of a culture medium, etc. are performed manually, and it is hard to say that efficient organization construction is performed. Thus, we suggest utility and possibility of applying microfluidics for formation of toroidal-like spheroid. Microfluidic devices to generate rotational flow were fabricated, and we conducted experiments using mouse embryo fibroblast cell.
AB - Spheroid is formed by making it agitate and circle in cell suspension. However, the injection of the cells used as pieces of spheroids, the recovery at the time of tissue formation, exchange of a culture medium, etc. are performed manually, and it is hard to say that efficient organization construction is performed. Thus, we suggest utility and possibility of applying microfluidics for formation of toroidal-like spheroid. Microfluidic devices to generate rotational flow were fabricated, and we conducted experiments using mouse embryo fibroblast cell.
UR - http://www.scopus.com/inward/record.url?scp=84961360271&partnerID=8YFLogxK
U2 - 10.1109/MHS.2014.7006134
DO - 10.1109/MHS.2014.7006134
M3 - Conference contribution
AN - SCOPUS:84961360271
T3 - 2014 International Symposium on Micro-NanoMechatronics and Human Science, MHS 2014
BT - 2014 International Symposium on Micro-NanoMechatronics and Human Science, MHS 2014
PB - Institute of Electrical and Electronics Engineers Inc.
T2 - 2014 International Symposium on Micro-NanoMechatronics and Human Science, MHS 2014
Y2 - 10 November 2014 through 12 November 2014
ER -