TY - GEN
T1 - Effect of N-terminal and C-terminal deletion of Cry1Ie of Bacillus thuringiensis on its expression and purification
AU - Shen, Huicong
AU - Zhang, Chunlu
AU - Guo, Shuyuan
AU - Zhang, Jie
AU - Song, Fuping
PY - 2009
Y1 - 2009
N2 - Crystal proteins are produced by the gram-positive bacterium Bacillus thuringiensis (Bt) during sporulation. The cry1Ie genes were silent in Bt strains, but can be overexpressed in Escherichia coli BL21 (DE3) in inclusion bodies, and the expressed product was toxic to Plutella xylostella, Ostrinia furnacalis, and soybean pod borer (Leguminivora glycinivorella). In order to know the N-terminal and C-terminal amino acid on the structure formation of Cry1Ie protein, four deletion mutants were constructed and the expression and purification of these mutants were studied.
AB - Crystal proteins are produced by the gram-positive bacterium Bacillus thuringiensis (Bt) during sporulation. The cry1Ie genes were silent in Bt strains, but can be overexpressed in Escherichia coli BL21 (DE3) in inclusion bodies, and the expressed product was toxic to Plutella xylostella, Ostrinia furnacalis, and soybean pod borer (Leguminivora glycinivorella). In order to know the N-terminal and C-terminal amino acid on the structure formation of Cry1Ie protein, four deletion mutants were constructed and the expression and purification of these mutants were studied.
KW - Bacillus thuringienesis
KW - Cry1Ie
KW - Insecticidal protein
KW - Mutant
UR - http://www.scopus.com/inward/record.url?scp=72749125820&partnerID=8YFLogxK
U2 - 10.1109/ICBBE.2009.5162705
DO - 10.1109/ICBBE.2009.5162705
M3 - Conference contribution
AN - SCOPUS:72749125820
SN - 9781424429028
T3 - 3rd International Conference on Bioinformatics and Biomedical Engineering, iCBBE 2009
BT - 3rd International Conference on Bioinformatics and Biomedical Engineering, iCBBE 2009
T2 - 3rd International Conference on Bioinformatics and Biomedical Engineering, iCBBE 2009
Y2 - 11 June 2009 through 13 June 2009
ER -