Determination of the Abundance Proteins of Adipose Synthesis and β-Oxidation Using 18O Isotope Labeled and QconCAT Protein Combined with Multiple Reaction Monitoring Mass Spectrometry

Chi Zhang; Jie Hong; Yong Qian Zhang; Yu Lin Deng

Determination of the Abundance Proteins of Adipose Synthesis and β-Oxidation Using ¹⁸O Isotope Labeled and QconCAT Protein Combined with Multiple Reaction Monitoring Mass Spectrometry

Chi Zhang, Jie Hong, Yong Qian Zhang^*, Yu Lin Deng

^*此作品的通讯作者

医学技术学院

Beijing Institute of Technology

科研成果: 期刊稿件 › 文章 › 同行评审

摘要

A novel method using QconCAT protein combined with ¹⁸O-labeling technique and multiple reaction monitoring mass spectrometry was established to quantify proteins involved in the pathway of adipose synthesis and β-oxidation. Firstly, the QconCAT proteins were constructed, and expressed in Escherichia coli. The digestion and the ¹⁸O labeling efficiency were optimized. Secondly, the novel method of quantifying adipose synthesis and β-oxidation was developed. Lastly, target proteins of obesity and control groups were quantified by MRM. The results showed that the abundance of the enoyl ACP reductase and malonyl-COA ACP transferase increased significantly in the obesity group with P<0.05. It is consistent to previous reports and means the method had its reliability to analyze the biological sample.

源语言	英语
页（从-至）	29-34
页数	6
期刊	Beijing Ligong Daxue Xuebao/Transaction of Beijing Institute of Technology
卷	37
出版状态	已出版 - 1 6月 2017

联合国可持续发展目标

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@article{8cad3c0b745548a9a122105669a0bee8,

title = "Determination of the Abundance Proteins of Adipose Synthesis and β-Oxidation Using 18O Isotope Labeled and QconCAT Protein Combined with Multiple Reaction Monitoring Mass Spectrometry",

abstract = "A novel method using QconCAT protein combined with 18O-labeling technique and multiple reaction monitoring mass spectrometry was established to quantify proteins involved in the pathway of adipose synthesis and β-oxidation. Firstly, the QconCAT proteins were constructed, and expressed in Escherichia coli. The digestion and the 18O labeling efficiency were optimized. Secondly, the novel method of quantifying adipose synthesis and β-oxidation was developed. Lastly, target proteins of obesity and control groups were quantified by MRM. The results showed that the abundance of the enoyl ACP reductase and malonyl-COA ACP transferase increased significantly in the obesity group with P<0.05. It is consistent to previous reports and means the method had its reliability to analyze the biological sample.",

keywords = "Concatamers of Q peptides, Multiple reaction monitoring, O labeling",

author = "Chi Zhang and Jie Hong and Zhang, {Yong Qian} and Deng, {Yu Lin}",

year = "2017",

month = jun,

day = "1",

language = "English",

volume = "37",

pages = "29--34",

journal = "Beijing Ligong Daxue Xuebao/Transaction of Beijing Institute of Technology",

issn = "1001-0645",

publisher = "Beijing Institute of Technology",

}

Determination of the Abundance Proteins of Adipose Synthesis and β-Oxidation Using ¹⁸O Isotope Labeled and QconCAT Protein Combined with Multiple Reaction Monitoring Mass Spectrometry. / Zhang, Chi; Hong, Jie; Zhang, Yong Qian 等.
在: Beijing Ligong Daxue Xuebao/Transaction of Beijing Institute of Technology, 卷 37, 01.06.2017, 页码 29-34.

科研成果: 期刊稿件 › 文章 › 同行评审

TY - JOUR

T1 - Determination of the Abundance Proteins of Adipose Synthesis and β-Oxidation Using 18O Isotope Labeled and QconCAT Protein Combined with Multiple Reaction Monitoring Mass Spectrometry

AU - Zhang, Chi

AU - Hong, Jie

AU - Zhang, Yong Qian

AU - Deng, Yu Lin

PY - 2017/6/1

Y1 - 2017/6/1

N2 - A novel method using QconCAT protein combined with 18O-labeling technique and multiple reaction monitoring mass spectrometry was established to quantify proteins involved in the pathway of adipose synthesis and β-oxidation. Firstly, the QconCAT proteins were constructed, and expressed in Escherichia coli. The digestion and the 18O labeling efficiency were optimized. Secondly, the novel method of quantifying adipose synthesis and β-oxidation was developed. Lastly, target proteins of obesity and control groups were quantified by MRM. The results showed that the abundance of the enoyl ACP reductase and malonyl-COA ACP transferase increased significantly in the obesity group with P<0.05. It is consistent to previous reports and means the method had its reliability to analyze the biological sample.

AB - A novel method using QconCAT protein combined with 18O-labeling technique and multiple reaction monitoring mass spectrometry was established to quantify proteins involved in the pathway of adipose synthesis and β-oxidation. Firstly, the QconCAT proteins were constructed, and expressed in Escherichia coli. The digestion and the 18O labeling efficiency were optimized. Secondly, the novel method of quantifying adipose synthesis and β-oxidation was developed. Lastly, target proteins of obesity and control groups were quantified by MRM. The results showed that the abundance of the enoyl ACP reductase and malonyl-COA ACP transferase increased significantly in the obesity group with P<0.05. It is consistent to previous reports and means the method had its reliability to analyze the biological sample.

KW - Concatamers of Q peptides

KW - Multiple reaction monitoring

KW - O labeling

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M3 - Article

AN - SCOPUS:85033672157

SN - 1001-0645

VL - 37

SP - 29

EP - 34

JO - Beijing Ligong Daxue Xuebao/Transaction of Beijing Institute of Technology

JF - Beijing Ligong Daxue Xuebao/Transaction of Beijing Institute of Technology

ER -

Determination of the Abundance Proteins of Adipose Synthesis and β-Oxidation Using 18O Isotope Labeled and QconCAT Protein Combined with Multiple Reaction Monitoring Mass Spectrometry

摘要

联合国可持续发展目标

其它文件与链接

指纹

引用此

Determination of the Abundance Proteins of Adipose Synthesis and β-Oxidation Using ¹⁸O Isotope Labeled and QconCAT Protein Combined with Multiple Reaction Monitoring Mass Spectrometry