Detection of 4 Relative Substances in Clarithromycin by HPLC

Li Quan Sun; Meng Nan Zhang; Bu Fang Ma; Guo Wei Yao; Ai Qin Luo

doi:10.15918/j.tbit1001-0645.2017.09.019

Detection of 4 Relative Substances in Clarithromycin by HPLC

Li Quan Sun, Meng Nan Zhang, Bu Fang Ma, Guo Wei Yao, Ai Qin Luo

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Beijing Institute of Technology

科研成果: 期刊稿件 › 文章 › 同行评审

摘要

An effective method was established and verified for the detection of clarithromycin and its relative substances J, M, N and K by HPLC. Clarithromycin relative substances sample was separated by isocratic elution with a Purospher STAR LP RP-18e C18 column and with the mobile phase 0.067 mol/L KH₂PO₄ solution (pH 4.0)/acetonitrile=60/40, the flow rate of 1.2 mL/min, the column temperature 35℃ and detection wavelength 205 nm, and the separation degrees were greater than 1.2. Analysis results show that, the method can provide good linearity (R>0.999) for all relative substances at the concentration range of 20~240 μg/mL, the average recovery rates keep at 87.80%~121.17%, its operation is simple and efficient, and can be able to simultaneously detect J, M, N and K in clarithromycin.

源语言	英语
页（从-至）	986-990
页数	5
期刊	Beijing Ligong Daxue Xuebao/Transaction of Beijing Institute of Technology
卷	37
期	9
DOI	https://doi.org/10.15918/j.tbit1001-0645.2017.09.019
出版状态	已出版 - 1 9月 2017

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title = "Detection of 4 Relative Substances in Clarithromycin by HPLC",

abstract = "An effective method was established and verified for the detection of clarithromycin and its relative substances J, M, N and K by HPLC. Clarithromycin relative substances sample was separated by isocratic elution with a Purospher STAR LP RP-18e C18 column and with the mobile phase 0.067 mol/L KH2PO4 solution (pH 4.0)/acetonitrile=60/40, the flow rate of 1.2 mL/min, the column temperature 35℃ and detection wavelength 205 nm, and the separation degrees were greater than 1.2. Analysis results show that, the method can provide good linearity (R>0.999) for all relative substances at the concentration range of 20~240 μg/mL, the average recovery rates keep at 87.80%~121.17%, its operation is simple and efficient, and can be able to simultaneously detect J, M, N and K in clarithromycin.",

keywords = "Clarithromycin, Detection, HPLC, Relative substances",

author = "Sun, {Li Quan} and Zhang, {Meng Nan} and Ma, {Bu Fang} and Yao, {Guo Wei} and Luo, {Ai Qin}",

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doi = "10.15918/j.tbit1001-0645.2017.09.019",

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T1 - Detection of 4 Relative Substances in Clarithromycin by HPLC

AU - Sun, Li Quan

AU - Zhang, Meng Nan

AU - Ma, Bu Fang

AU - Yao, Guo Wei

AU - Luo, Ai Qin

PY - 2017/9/1

Y1 - 2017/9/1

N2 - An effective method was established and verified for the detection of clarithromycin and its relative substances J, M, N and K by HPLC. Clarithromycin relative substances sample was separated by isocratic elution with a Purospher STAR LP RP-18e C18 column and with the mobile phase 0.067 mol/L KH2PO4 solution (pH 4.0)/acetonitrile=60/40, the flow rate of 1.2 mL/min, the column temperature 35℃ and detection wavelength 205 nm, and the separation degrees were greater than 1.2. Analysis results show that, the method can provide good linearity (R>0.999) for all relative substances at the concentration range of 20~240 μg/mL, the average recovery rates keep at 87.80%~121.17%, its operation is simple and efficient, and can be able to simultaneously detect J, M, N and K in clarithromycin.

AB - An effective method was established and verified for the detection of clarithromycin and its relative substances J, M, N and K by HPLC. Clarithromycin relative substances sample was separated by isocratic elution with a Purospher STAR LP RP-18e C18 column and with the mobile phase 0.067 mol/L KH2PO4 solution (pH 4.0)/acetonitrile=60/40, the flow rate of 1.2 mL/min, the column temperature 35℃ and detection wavelength 205 nm, and the separation degrees were greater than 1.2. Analysis results show that, the method can provide good linearity (R>0.999) for all relative substances at the concentration range of 20~240 μg/mL, the average recovery rates keep at 87.80%~121.17%, its operation is simple and efficient, and can be able to simultaneously detect J, M, N and K in clarithromycin.

KW - Clarithromycin

KW - Detection

KW - HPLC

KW - Relative substances

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JF - Beijing Ligong Daxue Xuebao/Transaction of Beijing Institute of Technology

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ER -

Detection of 4 Relative Substances in Clarithromycin by HPLC

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