TY - GEN
T1 - Control of flagellar motor using a real-time local environment chemical stimulation system
AU - Kojima, Masaru
AU - Motoyoshi, Takahiro
AU - Ohara, Kenichi
AU - Horade, Mitsuhiro
AU - Kamiyama, Kazuto
AU - Mae, Yasushi
AU - Arai, Tatsuo
N1 - Publisher Copyright:
© 2014 IEEE.
PY - 2014/9/22
Y1 - 2014/9/22
N2 - Single cell analysis has attracted much attention to reveal the localized biological information in detail. Local environmental control technique is developed to analyze the localized detail properties of single cells. In this paper, we propose the local environmental chemical stimulation system with micro dual pipettes to stimuli the local reagent concentration dynamically and automatically. Local environmental chemical stimulation by dual pipettes is applied to the rotational speed control of bacterial flagellar motor, which is a rotary molecular machine. Here, we show quick response and rotational speed control of Na-driven flagellar motor in both accelerating and relaxing directions was demonstrated by automatic switching the local spout between Na-containing and Na-free solutions with dual pipettes. It was shown that the rotational speed could be maintained by automatic controlling the spouting velocity of Na-containing and Na-free solution with multiplying the applied DC voltage. Furthermore, it was confirmed that by applying the P-control, rotational speed of flagellar motor could be controlled more stably.
AB - Single cell analysis has attracted much attention to reveal the localized biological information in detail. Local environmental control technique is developed to analyze the localized detail properties of single cells. In this paper, we propose the local environmental chemical stimulation system with micro dual pipettes to stimuli the local reagent concentration dynamically and automatically. Local environmental chemical stimulation by dual pipettes is applied to the rotational speed control of bacterial flagellar motor, which is a rotary molecular machine. Here, we show quick response and rotational speed control of Na-driven flagellar motor in both accelerating and relaxing directions was demonstrated by automatic switching the local spout between Na-containing and Na-free solutions with dual pipettes. It was shown that the rotational speed could be maintained by automatic controlling the spouting velocity of Na-containing and Na-free solution with multiplying the applied DC voltage. Furthermore, it was confirmed that by applying the P-control, rotational speed of flagellar motor could be controlled more stably.
UR - http://www.scopus.com/inward/record.url?scp=84929223790&partnerID=8YFLogxK
U2 - 10.1109/ICRA.2014.6907546
DO - 10.1109/ICRA.2014.6907546
M3 - Conference contribution
AN - SCOPUS:84929223790
T3 - Proceedings - IEEE International Conference on Robotics and Automation
SP - 4699
EP - 4704
BT - Proceedings - IEEE International Conference on Robotics and Automation
PB - Institute of Electrical and Electronics Engineers Inc.
T2 - 2014 IEEE International Conference on Robotics and Automation, ICRA 2014
Y2 - 31 May 2014 through 7 June 2014
ER -