Characterization of promoters in escherichia coli and application for xylitol synthesis

Cuiwei Wang, Zhe Li, Aamir Rasool, Hongnan Qu, Dazhang Dai, Chun Li*

*此作品的通讯作者

科研成果: 期刊稿件文章同行评审

4 引用 (Scopus)

摘要

Promoters are themost important tools to control and regulate the gene expression in synthetic biology and metabolic engineering. The expression of target genes in Escherichia coli is usually controlled by the high-strength inducible promoter with the result that the abnormally high transcription of these genes creates excessive metabolic load on the host, which decreases product formation. The constitutive expression systems are capable of avoiding these defects. In this study, to enrich the application of constitutive promoters in metabolic engineering, four promoters from the glycolytic pathway of E. coli were cloned and characterized using the enhanced green fluorescent protein as reporter. Among these promoters, PgapA was determined as the strongest one, the strength of which was about 8.92% of that of the widely used inducible promoter PT7. This promoter was used to control the expression of heterologous xylose reductase in E. coli for xylitol synthesis so as to verify its function in pathway engineering. Themaximumxylitol titer (40.6 g·L-1) produced by engineered E. coli under the control of the constitutive promoter PgapAwas obviously higher than that under the control of the inducible promoter PT7, indicating the feasibility and superiority of promoter PgapA in the metabolic engineering of E. coli.

源语言英语
页(从-至)234-240
页数7
期刊Chinese Journal of Chemical Engineering
23
1
DOI
出版状态已出版 - 2015

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