Characteristics and mechanism of cell apoptosis induced by high fluence low-power laser irradiation

Shengnan Wu, Da Xing*

*此作品的通讯作者

科研成果: 书/报告/会议事项章节会议稿件同行评审

摘要

High fluence low-power laser irradiation (LPLI) can induce cell apoptosis which is mediated by a high level of mitochondrial reactive oxygen species (ROS) production; however the mechanism is still unclear. Here, we further studied the mitochondrial signaling pathways involved in the apoptotic process. Activation of caspase-9 indicated an apoptotic process occurred under the high fluence LPLI treatment. Increasing of dichlorodihydrofluorescein diacetate (H2DCFDA) fluorescence products showed a high level of mitochondrial ROS generation after irradiation. Cyclosporine A (CsA) has been reported to inhibit some kinds of apoptosis, which are especially mediated by ROS. The question is whether CsA has some effect on high fluence LPLI induced apoptosis. Results showed that CsA significantly delayed mitochondria depolarization, observably delayed cell death in response to high fluence LPLI treatment demonstrating a significant protective role of CsA on the apoptotic process. These results suggest that high fluence LPLI induced cell apoptosis via some CsA-sensitive mitochondrial signal pathways.

源语言英语
主期刊名Biophotonics and Immune Responses III
DOI
出版状态已出版 - 2008
已对外发布
活动Biophotonics and Immune Responses III - San Jose, CA, 美国
期限: 21 1月 200821 1月 2008

出版系列

姓名Progress in Biomedical Optics and Imaging - Proceedings of SPIE
6857
ISSN(印刷版)1605-7422

会议

会议Biophotonics and Immune Responses III
国家/地区美国
San Jose, CA
时期21/01/0821/01/08

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