Automatic single cell transfer module

Akiyuki Hasegawa*, Huseyin Uvet, Kenichi Ohara, Tomohito Takubo, Yasushi Mae, Tatsuo Arai

*此作品的通讯作者

科研成果: 书/报告/会议事项章节会议稿件同行评审

摘要

In this paper, we presented a design for an automated single cell loading and supply system that can be used with complex microfluidic applications requiring single cell loading such as the current nuclear transplantation method. The aim of the system is to automatically transfer mammalian donor (∼15μm) or egg (∼100μm) cells one by one from a container to a PDMS micro-channel and then transport them to other modules. The system consists of two main parts; a single cell suction module, and a PDMS-based microfluidic chip controlled by an external pump. The desired number of vacuumed cells can be directed into the microfluidic chip and stored in a docking area. From the batch, they can be moved to next module by activating pneumatic pressure valves located on two sides of the chip. The entire mechanism is combined with monitoring systems that perform the detection/tracking and control program.

源语言英语
主期刊名2009 IEEE International Conference on Robotics and Biomimetics, ROBIO 2009
147-152
页数6
DOI
出版状态已出版 - 2009
已对外发布
活动2009 IEEE International Conference on Robotics and Biomimetics, ROBIO 2009 - Guilin, 中国
期限: 19 12月 200923 12月 2009

出版系列

姓名2009 IEEE International Conference on Robotics and Biomimetics, ROBIO 2009

会议

会议2009 IEEE International Conference on Robotics and Biomimetics, ROBIO 2009
国家/地区中国
Guilin
时期19/12/0923/12/09

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引用此

Hasegawa, A., Uvet, H., Ohara, K., Takubo, T., Mae, Y., & Arai, T. (2009). Automatic single cell transfer module. 在 2009 IEEE International Conference on Robotics and Biomimetics, ROBIO 2009 (页码 147-152). 文章 5420614 (2009 IEEE International Conference on Robotics and Biomimetics, ROBIO 2009). https://doi.org/10.1109/ROBIO.2009.5420614