Automated Tracking System with Head and Tail Recognition for Time-Lapse Observation of Free-Moving C. elegans

Shengnan Dong, Xiaoming Liu, Pengyun Li, Xiaoqing Tang, Dan Liu, Masaru Kojima, Qiang Huang, Tatsuo Arai

科研成果: 书/报告/会议事项章节会议稿件同行评审

2 引用 (Scopus)

摘要

In this paper, an automated tracking system with head and tail recognition for time-lapse observation of free-moving C. elegans is presented. In microscale field, active C. elegans can move out of the view easily without an automated tracking system because of the narrow field of view and rapid speed of C. elegans. In our previous works, we constructed an automated platform with 3D freedom to track centroid region of the nematode successfully. However, tracking time was not long enough to support a full time-lapse observation. Our proposed system in this study integrate the detection method in horizontal plane with depth evaluation more tightly. Tracking time and response speed have been greatly improved. Besides, we make full use of curvature calculation to make the system recognize the head and tail of C. elegans and the recognition rate can be up to 95%. The results demonstrate that the system can fully achieve automated long-term tracking of a free-living nematode and will be a nice tool for C. elegans behavioral analysis.

源语言英语
主期刊名2020 IEEE International Conference on Robotics and Automation, ICRA 2020
出版商Institute of Electrical and Electronics Engineers Inc.
9257-9262
页数6
ISBN(电子版)9781728173955
DOI
出版状态已出版 - 5月 2020
活动2020 IEEE International Conference on Robotics and Automation, ICRA 2020 - Paris, 法国
期限: 31 5月 202031 8月 2020

出版系列

姓名Proceedings - IEEE International Conference on Robotics and Automation
ISSN(印刷版)1050-4729

会议

会议2020 IEEE International Conference on Robotics and Automation, ICRA 2020
国家/地区法国
Paris
时期31/05/2031/08/20

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引用此

Dong, S., Liu, X., Li, P., Tang, X., Liu, D., Kojima, M., Huang, Q., & Arai, T. (2020). Automated Tracking System with Head and Tail Recognition for Time-Lapse Observation of Free-Moving C. elegans. 在 2020 IEEE International Conference on Robotics and Automation, ICRA 2020 (页码 9257-9262). 文章 9197546 (Proceedings - IEEE International Conference on Robotics and Automation). Institute of Electrical and Electronics Engineers Inc.. https://doi.org/10.1109/ICRA40945.2020.9197546