A novel ratiometric fluorescent probe with high selectivity for lysosomal nitric oxide imaging

Zhiling Xu, Songtao Liu, Liren Xu, Zichun Li, Xiaoli Zhang, Hao Kang, Yifan Liu, Jin Yu, Jing Jing*, Guangle Niu*, Xiaoling Zhang*

*此作品的通讯作者

科研成果: 期刊稿件文章同行评审

9 引用 (Scopus)

摘要

Nitric oxide (NO) plays critical roles in both physiology and pathology, serving as a significant signaling molecule. Recent investigations have uncovered the pivotal role of lysosome as a critical organelle where intracellular NO exists and takes function. In this study, we developed a novel ratiometric fluorescent probe called XL-NO and modified it with a morpholine unit, which followed the intramolecular charge transfer (ICT) mechanism. The probe could detect lysosomal nitric oxide with high selectivity and sensitivity. The probe XL-NO contained a secondary amine moiety that could readily react with NO in lysosomes, leading to the formation of the N-nitrosation product. The N-nitroso structure enhanced the capability in push-pull electron, which obviously led to the change of fluorescence from 621 nm to 521 nm. In addition, XL-NO was discovered to have some evident advantages, such as significant ratiometric signal (I521/I621) change, strong anti-interference ability, good biocompatibility, and a low detection limit (LOD = 44.3 nM), which were crucial for the detection of lysosomal NO. To evaluate the practical application of XL-NO, NO imaging experiments were performed in both living cells and zebrafish. The results from these experiments confirmed the feasibility and reliability of XL-NO for exogenous/endogenous NO imaging and lysosome targeting.

源语言英语
文章编号342303
期刊Analytica Chimica Acta
1297
DOI
出版状态已出版 - 8 4月 2024

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引用此

Xu, Z., Liu, S., Xu, L., Li, Z., Zhang, X., Kang, H., Liu, Y., Yu, J., Jing, J., Niu, G., & Zhang, X. (2024). A novel ratiometric fluorescent probe with high selectivity for lysosomal nitric oxide imaging. Analytica Chimica Acta, 1297, 文章 342303. https://doi.org/10.1016/j.aca.2024.342303