A fast mapping method in the ISAF reconstruction algorithm

Gongming Wang, Fa Zhang*, Qi Chu, Fei Sun, Zhiyong Liu

*此作品的通讯作者

科研成果: 书/报告/会议事项章节会议稿件同行评审

摘要

ISAF (icosahedral symmetry-adapted functions) algorithm is the new high-resolution algorithm of icosahedral molecules. But its running speed is very slow because of the time-consuming operations of mapping sampling points into 3D space. In this paper, a fast mapping method is proposed to increase the running speed of this stage. First of all, the angle corresponding to one pixel arc in the maximum Fourier ring was taken as the sampling angle and the same angle sampling was applied in every rings. After that, the sampling points in ring R1 were mapped into 3D space. Finally, the 3D spatial positions of radial sampling points in other rings were deduced according to the rotate angle invariability of radial sampling points. The simulated data of PSV-F (Penicillium stoloniferum virus F) and experimental Cryo-EM data of CPV (cytoplasmic polyhedrosis virus) were used for validating this method. The results show that the whole speedup reaches to an order of magnitude at the premise of assuring accuracy. In addition, the speedup is increasing with the increase of the maximum Fourier radius and the number of projections.

源语言英语
主期刊名33rd Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS 2011
3930-3933
页数4
DOI
出版状态已出版 - 2011
已对外发布
活动33rd Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS 2011 - Boston, MA, 美国
期限: 30 8月 20113 9月 2011

出版系列

姓名Proceedings of the Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS
ISSN(印刷版)1557-170X

会议

会议33rd Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS 2011
国家/地区美国
Boston, MA
时期30/08/113/09/11

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