辅因子NADPH调控对D-1, 2, 4-丁三醇合成的影响

D-1, 2, 4-Butanetriol (BT) is an important intermediate in organic synthesis.Cytosolic cofactor NADPH of recombinant Escherichia coli that is used for synthesis of BTO is disturbed by deletion of zwf encoding for 6-glucose phosphate dehydrogenase and pgi encoding for glucose isomerase, to investigate the effects of NADPH/NADP⁺ on BTO synthesis via recombinant Escherichia coli. The results show that cytosolic NADPH/NADP⁺ in MJ135kpG strain with pgi gene deleted increases by 25%, and the appropriate addition of glucose in the medium can enhance the supply of cytosolic NADPH, which can promote the synthesis of BTO.As the initial concentration of glucose is 2 g•L^-1, the yield of BTO reaches 4.02 g•L^-1 in shake flask, and further increases to 4.13 g•L^-1 by adding glucose 4 g•L^-1 at 24 h.In a sum, enhancing NADPH supply is an effective strategy to improve BTO yield.