乙酰胆碱酯酶纳米胶囊的合成与稳定性研究

Chengzhao Tu; Meiling Lei; Qingping Cheng; Lin Ye

doi:10.11730/j.issn.1001-7631.2020.04.0246.07

乙酰胆碱酯酶纳米胶囊的合成与稳定性研究

Chengzhao Tu, Meiling Lei^*, Qingping Cheng, Lin Ye

^*此作品的通讯作者

材料学院

科研成果: 期刊稿件 › 文章 › 同行评审

摘要

Acetylcholinesterase nanocapsules (nAChE) were prepared by two-step method. Firstly, n-acryloyloxysuccinimide was used to introduce double bonds on the surface of AChE. Then, N-(3-aminopropyl) methacrylate and acrylamide were used as monomers, and N,N'-methylene bisacrylamide was used as crosslinking agent to prepare AChE nanocapsules. The results of dynamic light scattering (DLS) showed that the particle size of AChE nanocapsules increased, indicating the successful preparation. The results of enzyme activity test showed that the nanocapsules could not only retain the catalytic activity of AChE, but also greatly improved its high temperature stability and organic solvent stability.

投稿的翻译标题	Synthesis and Stability of Acetylcholinesterase Nanocapsules
源语言	繁体中文
页（从-至）	246-252
页数	7
期刊	Huaxue Fanying Gongcheng Yu Gongyi/Chemical Reaction Engineering and Technology
卷	36
期	3
DOI	https://doi.org/10.11730/j.issn.1001-7631.2020.04.0246.07
出版状态	已出版 - 25 6月 2020

关键词

Acetylcholinesterase
In-situ radical polymerization
Nanocapsule
Organic solvent stability
Thermal stability

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10.11730/j.issn.1001-7631.2020.04.0246.07

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引用此

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abstract = "Acetylcholinesterase nanocapsules (nAChE) were prepared by two-step method. Firstly, n-acryloyloxysuccinimide was used to introduce double bonds on the surface of AChE. Then, N-(3-aminopropyl) methacrylate and acrylamide were used as monomers, and N,N'-methylene bisacrylamide was used as crosslinking agent to prepare AChE nanocapsules. The results of dynamic light scattering (DLS) showed that the particle size of AChE nanocapsules increased, indicating the successful preparation. The results of enzyme activity test showed that the nanocapsules could not only retain the catalytic activity of AChE, but also greatly improved its high temperature stability and organic solvent stability.",

keywords = "Acetylcholinesterase, In-situ radical polymerization, Nanocapsule, Organic solvent stability, Thermal stability",

author = "Chengzhao Tu and Meiling Lei and Qingping Cheng and Lin Ye",

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T1 - 乙酰胆碱酯酶纳米胶囊的合成与稳定性研究

AU - Tu, Chengzhao

AU - Lei, Meiling

AU - Cheng, Qingping

AU - Ye, Lin

PY - 2020/6/25

Y1 - 2020/6/25

N2 - Acetylcholinesterase nanocapsules (nAChE) were prepared by two-step method. Firstly, n-acryloyloxysuccinimide was used to introduce double bonds on the surface of AChE. Then, N-(3-aminopropyl) methacrylate and acrylamide were used as monomers, and N,N'-methylene bisacrylamide was used as crosslinking agent to prepare AChE nanocapsules. The results of dynamic light scattering (DLS) showed that the particle size of AChE nanocapsules increased, indicating the successful preparation. The results of enzyme activity test showed that the nanocapsules could not only retain the catalytic activity of AChE, but also greatly improved its high temperature stability and organic solvent stability.

AB - Acetylcholinesterase nanocapsules (nAChE) were prepared by two-step method. Firstly, n-acryloyloxysuccinimide was used to introduce double bonds on the surface of AChE. Then, N-(3-aminopropyl) methacrylate and acrylamide were used as monomers, and N,N'-methylene bisacrylamide was used as crosslinking agent to prepare AChE nanocapsules. The results of dynamic light scattering (DLS) showed that the particle size of AChE nanocapsules increased, indicating the successful preparation. The results of enzyme activity test showed that the nanocapsules could not only retain the catalytic activity of AChE, but also greatly improved its high temperature stability and organic solvent stability.

KW - Acetylcholinesterase

KW - In-situ radical polymerization

KW - Nanocapsule

KW - Organic solvent stability

KW - Thermal stability

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DO - 10.11730/j.issn.1001-7631.2020.04.0246.07

M3 - 文章

AN - SCOPUS:85105474137

SN - 1001-7631

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JO - Huaxue Fanying Gongcheng Yu Gongyi/Chemical Reaction Engineering and Technology

JF - Huaxue Fanying Gongcheng Yu Gongyi/Chemical Reaction Engineering and Technology

IS - 3

ER -

乙酰胆碱酯酶纳米胶囊的合成与稳定性研究

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