Structural deduction of pregnane glycosides from Dregea sinensis Hemsl by high-performance liquid chromatography and electrospray ionization-tandem mass spectrometry

Shaohua Jia; Juan Song; Rongji Dai; Yulin Deng; Fang Lv

doi:10.1016/j.ijms.2017.01.018

Structural deduction of pregnane glycosides from Dregea sinensis Hemsl by high-performance liquid chromatography and electrospray ionization-tandem mass spectrometry

Shaohua Jia, Juan Song, Rongji Dai, Yulin Deng, Fang Lv^*

^*Corresponding author for this work

Beijing Institute of Technology

Research output: Contribution to journal › Article › peer-review

6 Citations (Scopus)

Abstract

In the search for novel natural products in the plant, particularly those with potential bioactivity, the MS fragmentation behavior of nine pregnane glycosides previously isolated from Dregea sinensis Hemsl was investigated by electrospray ionization-multistage tandem mass spectrometry (ESI–MSⁿ) equipped with an ion trap analyzer. Furthermore, by high-performance liquid chromtography (HPLC) coupled to ESI–MSⁿ, the thirty-eight unknown pregnane glycosides, combined with six known pregnane glycosides were deduced on the basis of the fragmentation patterns observed from the crude EtOAc extract of D. sinensis Hemsl. This study shows that the HPLC-ESI–MSⁿ is an effective method to rapid and accurate online identification of pregnane glycosides from plant extracts.

Original language	English
Pages (from-to)	38-43
Number of pages	6
Journal	International Journal of Mass Spectrometry
Volume	415
DOIs	https://doi.org/10.1016/j.ijms.2017.01.018
Publication status	Published - 1 Apr 2017

Keywords

Dregea sinensis Hemsl
Electrospray ionization-tandem mass spectrometry
High-performance liquid chromatography
Pregnane glycosides

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10.1016/j.ijms.2017.01.018

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title = "Structural deduction of pregnane glycosides from Dregea sinensis Hemsl by high-performance liquid chromatography and electrospray ionization-tandem mass spectrometry",

abstract = "In the search for novel natural products in the plant, particularly those with potential bioactivity, the MS fragmentation behavior of nine pregnane glycosides previously isolated from Dregea sinensis Hemsl was investigated by electrospray ionization-multistage tandem mass spectrometry (ESI–MSn) equipped with an ion trap analyzer. Furthermore, by high-performance liquid chromtography (HPLC) coupled to ESI–MSn, the thirty-eight unknown pregnane glycosides, combined with six known pregnane glycosides were deduced on the basis of the fragmentation patterns observed from the crude EtOAc extract of D. sinensis Hemsl. This study shows that the HPLC-ESI–MSn is an effective method to rapid and accurate online identification of pregnane glycosides from plant extracts.",

keywords = "Dregea sinensis Hemsl, Electrospray ionization-tandem mass spectrometry, High-performance liquid chromatography, Pregnane glycosides",

author = "Shaohua Jia and Juan Song and Rongji Dai and Yulin Deng and Fang Lv",

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T1 - Structural deduction of pregnane glycosides from Dregea sinensis Hemsl by high-performance liquid chromatography and electrospray ionization-tandem mass spectrometry

AU - Jia, Shaohua

AU - Song, Juan

AU - Dai, Rongji

AU - Deng, Yulin

AU - Lv, Fang

PY - 2017/4/1

Y1 - 2017/4/1

N2 - In the search for novel natural products in the plant, particularly those with potential bioactivity, the MS fragmentation behavior of nine pregnane glycosides previously isolated from Dregea sinensis Hemsl was investigated by electrospray ionization-multistage tandem mass spectrometry (ESI–MSn) equipped with an ion trap analyzer. Furthermore, by high-performance liquid chromtography (HPLC) coupled to ESI–MSn, the thirty-eight unknown pregnane glycosides, combined with six known pregnane glycosides were deduced on the basis of the fragmentation patterns observed from the crude EtOAc extract of D. sinensis Hemsl. This study shows that the HPLC-ESI–MSn is an effective method to rapid and accurate online identification of pregnane glycosides from plant extracts.

AB - In the search for novel natural products in the plant, particularly those with potential bioactivity, the MS fragmentation behavior of nine pregnane glycosides previously isolated from Dregea sinensis Hemsl was investigated by electrospray ionization-multistage tandem mass spectrometry (ESI–MSn) equipped with an ion trap analyzer. Furthermore, by high-performance liquid chromtography (HPLC) coupled to ESI–MSn, the thirty-eight unknown pregnane glycosides, combined with six known pregnane glycosides were deduced on the basis of the fragmentation patterns observed from the crude EtOAc extract of D. sinensis Hemsl. This study shows that the HPLC-ESI–MSn is an effective method to rapid and accurate online identification of pregnane glycosides from plant extracts.

KW - Dregea sinensis Hemsl

KW - Electrospray ionization-tandem mass spectrometry

KW - High-performance liquid chromatography

KW - Pregnane glycosides

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DO - 10.1016/j.ijms.2017.01.018

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JF - International Journal of Mass Spectrometry

ER -

Structural deduction of pregnane glycosides from Dregea sinensis Hemsl by high-performance liquid chromatography and electrospray ionization-tandem mass spectrometry

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Keywords

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