Seperation and determination of glycyrrhizic acid and liquiritin in licorice using SPE-RP-HPLC

Yu Hong Yu*, Jian Jun Cai, Rong Ji Dai, Yu Lin Deng, Kun Yang, Wei Wei Meng

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

1 Citation (Scopus)

Abstract

Glycyrrhizic acid (GA) and liquiritin are the two classes of bioactive components in licorice with known pharmacological effects. In our stud, different methods were compared to separate GA an- d liquiritin. Solid-phase extraction (SPE) method was verified to separate GA and liquiritin effectively. The method is fast, easy, reliable, and inexpensive; especially in the quality control. Moreover, we built a new method to determine Glycyrrhizic acid and liquiritin simultaneously using reversed phase high performance liquid chromatography (RP-HPLC) In the HPLC analyze, gradient elution and two wavelength were used . The regression line for GA was y =15340.6x+12.81, the regression for liquiritin line was y =9269.1x+20.63, The recovery rates were 101.3% and 99.4%; RSD were 1.01% and 0.92%.

Original languageEnglish
Title of host publication2007 IEEE/ICME International Conference on Complex Medical Engineering, CME 2007
Pages1856-1860
Number of pages5
DOIs
Publication statusPublished - 2007
Event2007 IEEE/ICME International Conference on Complex Medical Engineering, CME 2007 - Beijing, China
Duration: 23 May 200727 May 2007

Publication series

Name2007 IEEE/ICME International Conference on Complex Medical Engineering, CME 2007

Conference

Conference2007 IEEE/ICME International Conference on Complex Medical Engineering, CME 2007
Country/TerritoryChina
CityBeijing
Period23/05/0727/05/07

Keywords

  • Glycyrrhizic acid
  • Liquiritin
  • RP-HPLC
  • Separation
  • Solid-phase extraction

Fingerprint

Dive into the research topics of 'Seperation and determination of glycyrrhizic acid and liquiritin in licorice using SPE-RP-HPLC'. Together they form a unique fingerprint.

Cite this