Selective separation and enrichment of proteins in aqueous two-phase extraction system

Feng Qu; Hao Qin; Min Dong; Dong Xu Zhao; Xin Ying Zhao; Jing Hua Zhang

doi:10.1016/j.cclet.2009.04.023

Selective separation and enrichment of proteins in aqueous two-phase extraction system

Feng Qu^*, Hao Qin, Min Dong, Dong Xu Zhao, Xin Ying Zhao, Jing Hua Zhang

^*Corresponding author for this work

School of Life Science

Research output: Contribution to journal › Article › peer-review

13 Citations (Scopus)

Abstract

A simple aqueous two-phase extraction system (ATPS) of PEG/phosphate was proposed for selective separation and enrichment of proteins. The combination of ATPE with HPLC was applied to identify the partition of proteins in two phases. Five proteins (bovine serum albumin, Cytochrome C, lysozyme, myoglobin, and trypsin) were used as model proteins to study the effect of phosphate concentration and pH on proteins partition. The PEG/phosphate system was firstly applied to real human saliva and plasma samples, some proteins showed obviously different partition in two phases. The primary results manifest the selective separation and enrichment of proteins in ATPS provided the potential for high abundance proteins depletion in proteomics.

Original language	English
Pages (from-to)	1100-1102
Number of pages	3
Journal	Chinese Chemical Letters
Volume	20
Issue number	9
DOIs	https://doi.org/10.1016/j.cclet.2009.04.023
Publication status	Published - Sept 2009

Keywords

Aqueous two-phase extraction
Proteins
Saliva and plasma
Selective separation and enrichment

Access to Document

10.1016/j.cclet.2009.04.023

Cite this

@article{7263b0595a124b128b0421159a08d2ad,

title = "Selective separation and enrichment of proteins in aqueous two-phase extraction system",

abstract = "A simple aqueous two-phase extraction system (ATPS) of PEG/phosphate was proposed for selective separation and enrichment of proteins. The combination of ATPE with HPLC was applied to identify the partition of proteins in two phases. Five proteins (bovine serum albumin, Cytochrome C, lysozyme, myoglobin, and trypsin) were used as model proteins to study the effect of phosphate concentration and pH on proteins partition. The PEG/phosphate system was firstly applied to real human saliva and plasma samples, some proteins showed obviously different partition in two phases. The primary results manifest the selective separation and enrichment of proteins in ATPS provided the potential for high abundance proteins depletion in proteomics.",

keywords = "Aqueous two-phase extraction, Proteins, Saliva and plasma, Selective separation and enrichment",

author = "Feng Qu and Hao Qin and Min Dong and Zhao, {Dong Xu} and Zhao, {Xin Ying} and Zhang, {Jing Hua}",

year = "2009",

month = sep,

doi = "10.1016/j.cclet.2009.04.023",

language = "English",

volume = "20",

pages = "1100--1102",

journal = "Chinese Chemical Letters",

issn = "1001-8417",

publisher = "Elsevier B.V.",

number = "9",

}

TY - JOUR

T1 - Selective separation and enrichment of proteins in aqueous two-phase extraction system

AU - Qu, Feng

AU - Qin, Hao

AU - Dong, Min

AU - Zhao, Dong Xu

AU - Zhao, Xin Ying

AU - Zhang, Jing Hua

PY - 2009/9

Y1 - 2009/9

N2 - A simple aqueous two-phase extraction system (ATPS) of PEG/phosphate was proposed for selective separation and enrichment of proteins. The combination of ATPE with HPLC was applied to identify the partition of proteins in two phases. Five proteins (bovine serum albumin, Cytochrome C, lysozyme, myoglobin, and trypsin) were used as model proteins to study the effect of phosphate concentration and pH on proteins partition. The PEG/phosphate system was firstly applied to real human saliva and plasma samples, some proteins showed obviously different partition in two phases. The primary results manifest the selective separation and enrichment of proteins in ATPS provided the potential for high abundance proteins depletion in proteomics.

AB - A simple aqueous two-phase extraction system (ATPS) of PEG/phosphate was proposed for selective separation and enrichment of proteins. The combination of ATPE with HPLC was applied to identify the partition of proteins in two phases. Five proteins (bovine serum albumin, Cytochrome C, lysozyme, myoglobin, and trypsin) were used as model proteins to study the effect of phosphate concentration and pH on proteins partition. The PEG/phosphate system was firstly applied to real human saliva and plasma samples, some proteins showed obviously different partition in two phases. The primary results manifest the selective separation and enrichment of proteins in ATPS provided the potential for high abundance proteins depletion in proteomics.

KW - Aqueous two-phase extraction

KW - Proteins

KW - Saliva and plasma

KW - Selective separation and enrichment

UR - http://www.scopus.com/inward/record.url?scp=67650403456&partnerID=8YFLogxK

U2 - 10.1016/j.cclet.2009.04.023

DO - 10.1016/j.cclet.2009.04.023

M3 - Article

AN - SCOPUS:67650403456

SN - 1001-8417

VL - 20

SP - 1100

EP - 1102

JO - Chinese Chemical Letters

JF - Chinese Chemical Letters

IS - 9

ER -

Selective separation and enrichment of proteins in aqueous two-phase extraction system

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Cite this