Polyethyleneimine-interlayered silica-core quantum dot-shell nanocomposites for sensitive detection of: Salmonella typhimurium via a lateral flow immunoassay

Bo Zhang; Xingsheng Yang; Xiaoxian Liu; Juan Li; Chongwen Wang; Shengqi Wang

doi:10.1039/c9ra09252h

Polyethyleneimine-interlayered silica-core quantum dot-shell nanocomposites for sensitive detection of: Salmonella typhimurium via a lateral flow immunoassay

Bo Zhang, Xingsheng Yang, Xiaoxian Liu, Juan Li^*, Chongwen Wang, Shengqi Wang

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

35 Citations (Scopus)

Abstract

Herein, we synthesized high-performance SiO₂-core quantum dot (QD)-shell nanocomposites (SiO₂@PEI-QDs) using the polyethyleneimine (PEI)-mediated adsorption method. Cationic PEI was used to form a positively charged interlayer on the SiO₂ core, which achieved a dense adsorption of carboxylated QDs to form a shell of QDs and maintained a good dispersibility of the nanocomposite. The SiO₂@PEI-QDs showed excellent stability and high luminescence, and served as high-performance fluorescent labels for the detection of bacteria when used with the lateral flow immunoassay (LFA) technique. An SiO₂@PEI-QD-based LFA strip was successfully applied to rapidly detect Salmonella typhimurium in milk samples with a low limit of 5 × 10² cells per mL.

Original language	English
Pages (from-to)	2483-2489
Number of pages	7
Journal	RSC Advances
Volume	10
Issue number	5
DOIs	https://doi.org/10.1039/c9ra09252h
Publication status	Published - 2020
Externally published	Yes

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title = "Polyethyleneimine-interlayered silica-core quantum dot-shell nanocomposites for sensitive detection of: Salmonella typhimurium via a lateral flow immunoassay",

abstract = "Herein, we synthesized high-performance SiO2-core quantum dot (QD)-shell nanocomposites (SiO2@PEI-QDs) using the polyethyleneimine (PEI)-mediated adsorption method. Cationic PEI was used to form a positively charged interlayer on the SiO2 core, which achieved a dense adsorption of carboxylated QDs to form a shell of QDs and maintained a good dispersibility of the nanocomposite. The SiO2@PEI-QDs showed excellent stability and high luminescence, and served as high-performance fluorescent labels for the detection of bacteria when used with the lateral flow immunoassay (LFA) technique. An SiO2@PEI-QD-based LFA strip was successfully applied to rapidly detect Salmonella typhimurium in milk samples with a low limit of 5 × 102 cells per mL.",

author = "Bo Zhang and Xingsheng Yang and Xiaoxian Liu and Juan Li and Chongwen Wang and Shengqi Wang",

note = "Publisher Copyright: {\textcopyright} 2020 The Royal Society of Chemistry.",

year = "2020",

doi = "10.1039/c9ra09252h",

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T1 - Polyethyleneimine-interlayered silica-core quantum dot-shell nanocomposites for sensitive detection of

T2 - Salmonella typhimurium via a lateral flow immunoassay

AU - Zhang, Bo

AU - Yang, Xingsheng

AU - Liu, Xiaoxian

AU - Li, Juan

AU - Wang, Chongwen

AU - Wang, Shengqi

PY - 2020

Y1 - 2020

N2 - Herein, we synthesized high-performance SiO2-core quantum dot (QD)-shell nanocomposites (SiO2@PEI-QDs) using the polyethyleneimine (PEI)-mediated adsorption method. Cationic PEI was used to form a positively charged interlayer on the SiO2 core, which achieved a dense adsorption of carboxylated QDs to form a shell of QDs and maintained a good dispersibility of the nanocomposite. The SiO2@PEI-QDs showed excellent stability and high luminescence, and served as high-performance fluorescent labels for the detection of bacteria when used with the lateral flow immunoassay (LFA) technique. An SiO2@PEI-QD-based LFA strip was successfully applied to rapidly detect Salmonella typhimurium in milk samples with a low limit of 5 × 102 cells per mL.

AB - Herein, we synthesized high-performance SiO2-core quantum dot (QD)-shell nanocomposites (SiO2@PEI-QDs) using the polyethyleneimine (PEI)-mediated adsorption method. Cationic PEI was used to form a positively charged interlayer on the SiO2 core, which achieved a dense adsorption of carboxylated QDs to form a shell of QDs and maintained a good dispersibility of the nanocomposite. The SiO2@PEI-QDs showed excellent stability and high luminescence, and served as high-performance fluorescent labels for the detection of bacteria when used with the lateral flow immunoassay (LFA) technique. An SiO2@PEI-QD-based LFA strip was successfully applied to rapidly detect Salmonella typhimurium in milk samples with a low limit of 5 × 102 cells per mL.

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DO - 10.1039/c9ra09252h

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JF - RSC Advances

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ER -

Polyethyleneimine-interlayered silica-core quantum dot-shell nanocomposites for sensitive detection of: Salmonella typhimurium via a lateral flow immunoassay

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