Non-blinking, highly luminescent, pH- and heavy-metal-ion-stable organic nanodots for bio-imaging

Fei Fei An; Jun Ye; Jin Feng Zhang; Yin Long Yang; Cai Jun Zheng; Xiu Juan Zhang; Zhuang Liu; Chun Sing Lee; Xiao Hong Zhang

doi:10.1039/c3tb20271b

Non-blinking, highly luminescent, pH- and heavy-metal-ion-stable organic nanodots for bio-imaging

Fei Fei An, Jun Ye, Jin Feng Zhang, Yin Long Yang, Cai Jun Zheng, Xiu Juan Zhang^*, Zhuang Liu, Chun Sing Lee, Xiao Hong Zhang

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

28 Citations (Scopus)

Abstract

Heavy-metal-free, organic-small-molecule-based fluorescent nanodots (Sdots, 31-60 nm in diameter) based on 2,7-di(4-(diphenylamino)phenyl-2,1,3- benzothiadiazol-7-yl)-9,9′-spirobifluorene (Spiro-BTA) were prepared through a simple solution process. The Sdots show not only non-blinking and high-brightness fluorescence but also stability in various pH conditions and heavy metal ion solutions. More importantly, Spiro-BTA Sdots demonstrate obviously much higher brightness with very high signal-to-background ratio in Hela cell, compared with common CdSe/ZnS QDs. In addition, they exhibit large Stokes shifts, broad absorption spectra, and low toxicity to living cells which enable their applications as good fluorescence probes for bio-imaging. Further application of Sdots for folate receptor-mediated live-cell endocytosis was demonstrated by non-convalent modification with folic acid linked multidentate ligands.

Original language	English
Pages (from-to)	3144-3151
Number of pages	8
Journal	Journal of Materials Chemistry B
Volume	1
Issue number	25
DOIs	https://doi.org/10.1039/c3tb20271b
Publication status	Published - 7 Jul 2013
Externally published	Yes

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title = "Non-blinking, highly luminescent, pH- and heavy-metal-ion-stable organic nanodots for bio-imaging",

abstract = "Heavy-metal-free, organic-small-molecule-based fluorescent nanodots (Sdots, 31-60 nm in diameter) based on 2,7-di(4-(diphenylamino)phenyl-2,1,3- benzothiadiazol-7-yl)-9,9′-spirobifluorene (Spiro-BTA) were prepared through a simple solution process. The Sdots show not only non-blinking and high-brightness fluorescence but also stability in various pH conditions and heavy metal ion solutions. More importantly, Spiro-BTA Sdots demonstrate obviously much higher brightness with very high signal-to-background ratio in Hela cell, compared with common CdSe/ZnS QDs. In addition, they exhibit large Stokes shifts, broad absorption spectra, and low toxicity to living cells which enable their applications as good fluorescence probes for bio-imaging. Further application of Sdots for folate receptor-mediated live-cell endocytosis was demonstrated by non-convalent modification with folic acid linked multidentate ligands.",

author = "An, {Fei Fei} and Jun Ye and Zhang, {Jin Feng} and Yang, {Yin Long} and Zheng, {Cai Jun} and Zhang, {Xiu Juan} and Zhuang Liu and Lee, {Chun Sing} and Zhang, {Xiao Hong}",

year = "2013",

month = jul,

day = "7",

doi = "10.1039/c3tb20271b",

language = "English",

volume = "1",

pages = "3144--3151",

journal = "Journal of Materials Chemistry B",

issn = "2050-7518",

publisher = "Royal Society of Chemistry",

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TY - JOUR

T1 - Non-blinking, highly luminescent, pH- and heavy-metal-ion-stable organic nanodots for bio-imaging

AU - An, Fei Fei

AU - Ye, Jun

AU - Zhang, Jin Feng

AU - Yang, Yin Long

AU - Zheng, Cai Jun

AU - Zhang, Xiu Juan

AU - Liu, Zhuang

AU - Lee, Chun Sing

AU - Zhang, Xiao Hong

PY - 2013/7/7

Y1 - 2013/7/7

N2 - Heavy-metal-free, organic-small-molecule-based fluorescent nanodots (Sdots, 31-60 nm in diameter) based on 2,7-di(4-(diphenylamino)phenyl-2,1,3- benzothiadiazol-7-yl)-9,9′-spirobifluorene (Spiro-BTA) were prepared through a simple solution process. The Sdots show not only non-blinking and high-brightness fluorescence but also stability in various pH conditions and heavy metal ion solutions. More importantly, Spiro-BTA Sdots demonstrate obviously much higher brightness with very high signal-to-background ratio in Hela cell, compared with common CdSe/ZnS QDs. In addition, they exhibit large Stokes shifts, broad absorption spectra, and low toxicity to living cells which enable their applications as good fluorescence probes for bio-imaging. Further application of Sdots for folate receptor-mediated live-cell endocytosis was demonstrated by non-convalent modification with folic acid linked multidentate ligands.

AB - Heavy-metal-free, organic-small-molecule-based fluorescent nanodots (Sdots, 31-60 nm in diameter) based on 2,7-di(4-(diphenylamino)phenyl-2,1,3- benzothiadiazol-7-yl)-9,9′-spirobifluorene (Spiro-BTA) were prepared through a simple solution process. The Sdots show not only non-blinking and high-brightness fluorescence but also stability in various pH conditions and heavy metal ion solutions. More importantly, Spiro-BTA Sdots demonstrate obviously much higher brightness with very high signal-to-background ratio in Hela cell, compared with common CdSe/ZnS QDs. In addition, they exhibit large Stokes shifts, broad absorption spectra, and low toxicity to living cells which enable their applications as good fluorescence probes for bio-imaging. Further application of Sdots for folate receptor-mediated live-cell endocytosis was demonstrated by non-convalent modification with folic acid linked multidentate ligands.

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U2 - 10.1039/c3tb20271b

DO - 10.1039/c3tb20271b

M3 - Article

AN - SCOPUS:84880164993

SN - 2050-7518

VL - 1

SP - 3144

EP - 3151

JO - Journal of Materials Chemistry B

JF - Journal of Materials Chemistry B

IS - 25

ER -

Non-blinking, highly luminescent, pH- and heavy-metal-ion-stable organic nanodots for bio-imaging

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