Microfluidic Chip Integrated with Hydrogel Microparticles and CdS Cation Interfacial Exchange for the Sensitive Determination of miRNA

Yimeng Zhao; Xuefei Lv; Zhao Peng; Kexin Zhao; Di Zhou; Yulin Deng

doi:10.1080/00032719.2023.2180803

Microfluidic Chip Integrated with Hydrogel Microparticles and CdS Cation Interfacial Exchange for the Sensitive Determination of miRNA

Yimeng Zhao, Xuefei Lv^*, Zhao Peng, Kexin Zhao, Di Zhou, Yulin Deng^*

^*Corresponding author for this work

School of Medical and Technology

Beijing Institute of Technology

Research output: Contribution to journal › Article › peer-review

1 Citation (Scopus)

Abstract

Abnormally expressed miRNAs have been employed as crucial indicators for the diagnosis and prognosis of disease. It is essential to develop quick, affordable, accurate, and multiplexed methods for miRNA detection. In this study, polyethylene glycol (PEG) hydrogel microparticles were used as solid-phase carriers for the hybridization chain reaction (HCR) in combination with cation-exchange fluorescence amplification, thus enabling the development of a new method for miRNA determination. The assay was sensitive with the detection limit for miRNA let7a equal to 46 pM. The assay was specific, the base-mismatched sequences of miRNA let7a were distinguished, and the serum matrix did not cause any interference. Moreover, by integration with a microfluidic chip, the throughput was significantly enhanced and the process was noticeably simplified. With further development, the developed method may be suitable for practical clinical analysis.

Original language	English
Pages (from-to)	2677-2691
Number of pages	15
Journal	Analytical Letters
Volume	56
Issue number	16
DOIs	https://doi.org/10.1080/00032719.2023.2180803
Publication status	Published - 2023

Keywords

Cation interfacial exchange
fluorescence
hybridization chain reaction
hydrogel
miRNA
microfluidic chip

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10.1080/00032719.2023.2180803

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title = "Microfluidic Chip Integrated with Hydrogel Microparticles and CdS Cation Interfacial Exchange for the Sensitive Determination of miRNA",

abstract = "Abnormally expressed miRNAs have been employed as crucial indicators for the diagnosis and prognosis of disease. It is essential to develop quick, affordable, accurate, and multiplexed methods for miRNA detection. In this study, polyethylene glycol (PEG) hydrogel microparticles were used as solid-phase carriers for the hybridization chain reaction (HCR) in combination with cation-exchange fluorescence amplification, thus enabling the development of a new method for miRNA determination. The assay was sensitive with the detection limit for miRNA let7a equal to 46 pM. The assay was specific, the base-mismatched sequences of miRNA let7a were distinguished, and the serum matrix did not cause any interference. Moreover, by integration with a microfluidic chip, the throughput was significantly enhanced and the process was noticeably simplified. With further development, the developed method may be suitable for practical clinical analysis.",

keywords = "Cation interfacial exchange, fluorescence, hybridization chain reaction, hydrogel, miRNA, microfluidic chip",

author = "Yimeng Zhao and Xuefei Lv and Zhao Peng and Kexin Zhao and Di Zhou and Yulin Deng",

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year = "2023",

doi = "10.1080/00032719.2023.2180803",

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TY - JOUR

T1 - Microfluidic Chip Integrated with Hydrogel Microparticles and CdS Cation Interfacial Exchange for the Sensitive Determination of miRNA

AU - Zhao, Yimeng

AU - Lv, Xuefei

AU - Peng, Zhao

AU - Zhao, Kexin

AU - Zhou, Di

AU - Deng, Yulin

PY - 2023

Y1 - 2023

N2 - Abnormally expressed miRNAs have been employed as crucial indicators for the diagnosis and prognosis of disease. It is essential to develop quick, affordable, accurate, and multiplexed methods for miRNA detection. In this study, polyethylene glycol (PEG) hydrogel microparticles were used as solid-phase carriers for the hybridization chain reaction (HCR) in combination with cation-exchange fluorescence amplification, thus enabling the development of a new method for miRNA determination. The assay was sensitive with the detection limit for miRNA let7a equal to 46 pM. The assay was specific, the base-mismatched sequences of miRNA let7a were distinguished, and the serum matrix did not cause any interference. Moreover, by integration with a microfluidic chip, the throughput was significantly enhanced and the process was noticeably simplified. With further development, the developed method may be suitable for practical clinical analysis.

AB - Abnormally expressed miRNAs have been employed as crucial indicators for the diagnosis and prognosis of disease. It is essential to develop quick, affordable, accurate, and multiplexed methods for miRNA detection. In this study, polyethylene glycol (PEG) hydrogel microparticles were used as solid-phase carriers for the hybridization chain reaction (HCR) in combination with cation-exchange fluorescence amplification, thus enabling the development of a new method for miRNA determination. The assay was sensitive with the detection limit for miRNA let7a equal to 46 pM. The assay was specific, the base-mismatched sequences of miRNA let7a were distinguished, and the serum matrix did not cause any interference. Moreover, by integration with a microfluidic chip, the throughput was significantly enhanced and the process was noticeably simplified. With further development, the developed method may be suitable for practical clinical analysis.

KW - Cation interfacial exchange

KW - fluorescence

KW - hybridization chain reaction

KW - hydrogel

KW - miRNA

KW - microfluidic chip

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U2 - 10.1080/00032719.2023.2180803

DO - 10.1080/00032719.2023.2180803

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SN - 0003-2719

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JO - Analytical Letters

JF - Analytical Letters

IS - 16

ER -

Microfluidic Chip Integrated with Hydrogel Microparticles and CdS Cation Interfacial Exchange for the Sensitive Determination of miRNA

Abstract

Keywords

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