Microchip based and immunochromatographic strip assays for the visual detection of interleukin-6 and of tumor necrosis factor α using gold nanoparticles as labels

Yan Man; Xuefei Lv; Javed Iqbal; Guang Peng; Da Song; Congxiao Zhang; Yulin Deng

doi:10.1007/s00604-014-1362-y

Microchip based and immunochromatographic strip assays for the visual detection of interleukin-6 and of tumor necrosis factor α using gold nanoparticles as labels

Yan Man, Xuefei Lv^*, Javed Iqbal, Guang Peng, Da Song, Congxiao Zhang, Yulin Deng

^*Corresponding author for this work

School of Medical and Technology

Beijing Institute of Technology

Research output: Contribution to journal › Article › peer-review

27 Citations (Scopus)

Abstract

The enzyme-linked immunesorbent assay (ELISA) is most frequently used for the determination of interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α). However, this technique is time-consuming and requires a substantial sample volume. We have developed (a) a new kind of colloidal gold-based immunochromatographic strip, and (b) a colloidal gold-based immunochromatographic microchip for simultaneous, rapid, and visual detection of IL-6 and TNF-α. The lowest limit of detection is 62.5 ng mL⁻¹ for IL-6, and 30 ng mL⁻¹ for TNF-α. Both the strip method and the microchip method are highly specific, user-friendly, and can be read out without the need for instrumentation.

Original language	English
Pages (from-to)	597-604
Number of pages	8
Journal	Microchimica Acta
Volume	182
Issue number	3-4
DOIs	https://doi.org/10.1007/s00604-014-1362-y
Publication status	Published - Feb 2014

Keywords

IL-6
Immunochromatographic microchip
Immunochromatographic strip
Simultaneous detection
TNF-α

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10.1007/s00604-014-1362-y

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title = "Microchip based and immunochromatographic strip assays for the visual detection of interleukin-6 and of tumor necrosis factor α using gold nanoparticles as labels",

abstract = "The enzyme-linked immunesorbent assay (ELISA) is most frequently used for the determination of interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α). However, this technique is time-consuming and requires a substantial sample volume. We have developed (a) a new kind of colloidal gold-based immunochromatographic strip, and (b) a colloidal gold-based immunochromatographic microchip for simultaneous, rapid, and visual detection of IL-6 and TNF-α. The lowest limit of detection is 62.5 ng mL−1 for IL-6, and 30 ng mL−1 for TNF-α. Both the strip method and the microchip method are highly specific, user-friendly, and can be read out without the need for instrumentation.",

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AU - Man, Yan

AU - Lv, Xuefei

AU - Iqbal, Javed

AU - Peng, Guang

AU - Song, Da

AU - Zhang, Congxiao

AU - Deng, Yulin

PY - 2014/2

Y1 - 2014/2

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AB - The enzyme-linked immunesorbent assay (ELISA) is most frequently used for the determination of interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α). However, this technique is time-consuming and requires a substantial sample volume. We have developed (a) a new kind of colloidal gold-based immunochromatographic strip, and (b) a colloidal gold-based immunochromatographic microchip for simultaneous, rapid, and visual detection of IL-6 and TNF-α. The lowest limit of detection is 62.5 ng mL−1 for IL-6, and 30 ng mL−1 for TNF-α. Both the strip method and the microchip method are highly specific, user-friendly, and can be read out without the need for instrumentation.

KW - IL-6

KW - Immunochromatographic microchip

KW - Immunochromatographic strip

KW - Simultaneous detection

KW - TNF-α

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Microchip based and immunochromatographic strip assays for the visual detection of interleukin-6 and of tumor necrosis factor α using gold nanoparticles as labels

Abstract

Keywords

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