Intravital imaging of tumor apoptosis with FRET probes during tumor therapy

Feifan Zhou; Da Xing; Shengnan Wu; Wei R. Chen

doi:10.1007/s11307-009-0235-y

Intravital imaging of tumor apoptosis with FRET probes during tumor therapy

Feifan Zhou, Da Xing^*, Shengnan Wu, Wei R. Chen

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

28 Citations (Scopus)

Abstract

Purpose: The aim of the study is to dynamically and non-invasively monitor the apoptosis events in vivo during photodynamic therapy (PDT) and chemotherapy. Procedures: A FRET probe, SCAT3, was utilized to determine activation of caspase-3 during tumor cell apoptosis in mice, induced by PDT, and cisplatin treatments. Using this method, dynamics of caspase-3 activation was observed both in vitro and in vivo. Results: Analysis of the fluorescent missions from tumor cells indicated that the caspase-3 activation started immediately after PDT treatment. In contrast, the caspase-3 activation started about 13 and 36 h after cisplatin treatment in vitro and in vivo, respectively. Conclusions: FRET could be used effectively to monitor activation of caspase-3 in living organism. This method could be used to provide rapid assessment of apoptosis induced by anti-tumor therapies for improvement of treatment efficacy.

Original language	English
Pages (from-to)	63-70
Number of pages	8
Journal	Molecular Imaging and Biology
Volume	12
Issue number	1
DOIs	https://doi.org/10.1007/s11307-009-0235-y
Publication status	Published - Feb 2010
Externally published	Yes

Keywords

Apoptosis
Caspase-3
Cisplatin
FRET
PDT
SCAT3

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1007/s11307-009-0235-y

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Zhou, F., Xing, D., Wu, S., & Chen, W. R. (2010). Intravital imaging of tumor apoptosis with FRET probes during tumor therapy. Molecular Imaging and Biology, 12(1), 63-70. https://doi.org/10.1007/s11307-009-0235-y

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abstract = "Purpose: The aim of the study is to dynamically and non-invasively monitor the apoptosis events in vivo during photodynamic therapy (PDT) and chemotherapy. Procedures: A FRET probe, SCAT3, was utilized to determine activation of caspase-3 during tumor cell apoptosis in mice, induced by PDT, and cisplatin treatments. Using this method, dynamics of caspase-3 activation was observed both in vitro and in vivo. Results: Analysis of the fluorescent missions from tumor cells indicated that the caspase-3 activation started immediately after PDT treatment. In contrast, the caspase-3 activation started about 13 and 36 h after cisplatin treatment in vitro and in vivo, respectively. Conclusions: FRET could be used effectively to monitor activation of caspase-3 in living organism. This method could be used to provide rapid assessment of apoptosis induced by anti-tumor therapies for improvement of treatment efficacy.",

keywords = "Apoptosis, Caspase-3, Cisplatin, FRET, PDT, SCAT3",

author = "Feifan Zhou and Da Xing and Shengnan Wu and Chen, {Wei R.}",

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T1 - Intravital imaging of tumor apoptosis with FRET probes during tumor therapy

AU - Zhou, Feifan

AU - Xing, Da

AU - Wu, Shengnan

AU - Chen, Wei R.

PY - 2010/2

Y1 - 2010/2

N2 - Purpose: The aim of the study is to dynamically and non-invasively monitor the apoptosis events in vivo during photodynamic therapy (PDT) and chemotherapy. Procedures: A FRET probe, SCAT3, was utilized to determine activation of caspase-3 during tumor cell apoptosis in mice, induced by PDT, and cisplatin treatments. Using this method, dynamics of caspase-3 activation was observed both in vitro and in vivo. Results: Analysis of the fluorescent missions from tumor cells indicated that the caspase-3 activation started immediately after PDT treatment. In contrast, the caspase-3 activation started about 13 and 36 h after cisplatin treatment in vitro and in vivo, respectively. Conclusions: FRET could be used effectively to monitor activation of caspase-3 in living organism. This method could be used to provide rapid assessment of apoptosis induced by anti-tumor therapies for improvement of treatment efficacy.

AB - Purpose: The aim of the study is to dynamically and non-invasively monitor the apoptosis events in vivo during photodynamic therapy (PDT) and chemotherapy. Procedures: A FRET probe, SCAT3, was utilized to determine activation of caspase-3 during tumor cell apoptosis in mice, induced by PDT, and cisplatin treatments. Using this method, dynamics of caspase-3 activation was observed both in vitro and in vivo. Results: Analysis of the fluorescent missions from tumor cells indicated that the caspase-3 activation started immediately after PDT treatment. In contrast, the caspase-3 activation started about 13 and 36 h after cisplatin treatment in vitro and in vivo, respectively. Conclusions: FRET could be used effectively to monitor activation of caspase-3 in living organism. This method could be used to provide rapid assessment of apoptosis induced by anti-tumor therapies for improvement of treatment efficacy.

KW - Apoptosis

KW - Caspase-3

KW - Cisplatin

KW - FRET

KW - PDT

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ER -

Intravital imaging of tumor apoptosis with FRET probes during tumor therapy

Abstract

Keywords

UN SDGs

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