Intravital imaging of tumor apoptosis with FRET probes during tumor therapy

Feifan Zhou, Da Xing*, Shengnan Wu, Wei R. Chen

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

28 Citations (Scopus)

Abstract

Purpose: The aim of the study is to dynamically and non-invasively monitor the apoptosis events in vivo during photodynamic therapy (PDT) and chemotherapy. Procedures: A FRET probe, SCAT3, was utilized to determine activation of caspase-3 during tumor cell apoptosis in mice, induced by PDT, and cisplatin treatments. Using this method, dynamics of caspase-3 activation was observed both in vitro and in vivo. Results: Analysis of the fluorescent missions from tumor cells indicated that the caspase-3 activation started immediately after PDT treatment. In contrast, the caspase-3 activation started about 13 and 36 h after cisplatin treatment in vitro and in vivo, respectively. Conclusions: FRET could be used effectively to monitor activation of caspase-3 in living organism. This method could be used to provide rapid assessment of apoptosis induced by anti-tumor therapies for improvement of treatment efficacy.

Original languageEnglish
Pages (from-to)63-70
Number of pages8
JournalMolecular Imaging and Biology
Volume12
Issue number1
DOIs
Publication statusPublished - Feb 2010
Externally publishedYes

Keywords

  • Apoptosis
  • Caspase-3
  • Cisplatin
  • FRET
  • PDT
  • SCAT3

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Zhou, F., Xing, D., Wu, S., & Chen, W. R. (2010). Intravital imaging of tumor apoptosis with FRET probes during tumor therapy. Molecular Imaging and Biology, 12(1), 63-70. https://doi.org/10.1007/s11307-009-0235-y