Abstract
Purpose: The aim of the study is to dynamically and non-invasively monitor the apoptosis events in vivo during photodynamic therapy (PDT) and chemotherapy. Procedures: A FRET probe, SCAT3, was utilized to determine activation of caspase-3 during tumor cell apoptosis in mice, induced by PDT, and cisplatin treatments. Using this method, dynamics of caspase-3 activation was observed both in vitro and in vivo. Results: Analysis of the fluorescent missions from tumor cells indicated that the caspase-3 activation started immediately after PDT treatment. In contrast, the caspase-3 activation started about 13 and 36 h after cisplatin treatment in vitro and in vivo, respectively. Conclusions: FRET could be used effectively to monitor activation of caspase-3 in living organism. This method could be used to provide rapid assessment of apoptosis induced by anti-tumor therapies for improvement of treatment efficacy.
Original language | English |
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Pages (from-to) | 63-70 |
Number of pages | 8 |
Journal | Molecular Imaging and Biology |
Volume | 12 |
Issue number | 1 |
DOIs | |
Publication status | Published - Feb 2010 |
Externally published | Yes |
Keywords
- Apoptosis
- Caspase-3
- Cisplatin
- FRET
- PDT
- SCAT3