Immobilization of trypsin onto artificial membrane for the possible application in the digestion reactor of proteins

Yueying Liu; Rong Ji Dai; Feng Qu; Weiwei Meng; Yulin Deng

doi:10.1109/ICCME.2007.4382054

Immobilization of trypsin onto artificial membrane for the possible application in the digestion reactor of proteins

Yueying Liu, Rong Ji Dai, Feng Qu, Weiwei Meng, Yulin Deng^*

^*Corresponding author for this work

Beijing Institute of Technology

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

Abstract

This paper described a novel method to immobilize trypsin on IAM. The immobilization procedures involved the following steps: IAM was packed into the fused silica capillary (400um I.D.×690μm O.D.×5cm length); subsequently, trypsin was dynamically immobilized on IAM by injecting 1mg/ml trypsin aqueous solution using the frontal chromatography. The activity of trypsin-micro-IMERs was on-line measured using BAEE (N-benzoyl-L-arginine ethyl ester) as the substrate of trysin. The results showed that the quantity of product BA and BAEE concentration increased linearly. The results illustrated that the quantity of BA at flow rate of 3 ul/min was obviously larger than that at flow rate of 6 ul/min. The bioactivity of immobilized trypsin was at least 69.3% in 24h.

Original language	English
Title of host publication	2007 IEEE/ICME International Conference on Complex Medical Engineering, CME 2007
Pages	1782-1786
Number of pages	5
DOIs	https://doi.org/10.1109/ICCME.2007.4382054
Publication status	Published - 2007
Event	2007 IEEE/ICME International Conference on Complex Medical Engineering, CME 2007 - Beijing, China Duration: 23 May 2007 → 27 May 2007

Publication series

Name	2007 IEEE/ICME International Conference on Complex Medical Engineering, CME 2007

Conference

Conference	2007 IEEE/ICME International Conference on Complex Medical Engineering, CME 2007
Country/Territory	China
City	Beijing
Period	23/05/07 → 27/05/07

Keywords

BAEE
Dynamica limmobilization
Trypsin-micro-IMERs

Access to Document

10.1109/ICCME.2007.4382054

Cite this

Liu, Y., Dai, R. J., Qu, F., Meng, W., & Deng, Y. (2007). Immobilization of trypsin onto artificial membrane for the possible application in the digestion reactor of proteins. In 2007 IEEE/ICME International Conference on Complex Medical Engineering, CME 2007 (pp. 1782-1786). Article 4382054 (2007 IEEE/ICME International Conference on Complex Medical Engineering, CME 2007). https://doi.org/10.1109/ICCME.2007.4382054

@inproceedings{42193c6283ab4136beed2fe79ea7ba34,

title = "Immobilization of trypsin onto artificial membrane for the possible application in the digestion reactor of proteins",

abstract = "This paper described a novel method to immobilize trypsin on IAM. The immobilization procedures involved the following steps: IAM was packed into the fused silica capillary (400um I.D.×690μm O.D.×5cm length); subsequently, trypsin was dynamically immobilized on IAM by injecting 1mg/ml trypsin aqueous solution using the frontal chromatography. The activity of trypsin-micro-IMERs was on-line measured using BAEE (N-benzoyl-L-arginine ethyl ester) as the substrate of trysin. The results showed that the quantity of product BA and BAEE concentration increased linearly. The results illustrated that the quantity of BA at flow rate of 3 ul/min was obviously larger than that at flow rate of 6 ul/min. The bioactivity of immobilized trypsin was at least 69.3% in 24h.",

keywords = "BAEE, Dynamica limmobilization, Trypsin-micro-IMERs",

author = "Yueying Liu and Dai, {Rong Ji} and Feng Qu and Weiwei Meng and Yulin Deng",

year = "2007",

doi = "10.1109/ICCME.2007.4382054",

language = "English",

isbn = "1424410789",

series = "2007 IEEE/ICME International Conference on Complex Medical Engineering, CME 2007",

pages = "1782--1786",

booktitle = "2007 IEEE/ICME International Conference on Complex Medical Engineering, CME 2007",

note = "2007 IEEE/ICME International Conference on Complex Medical Engineering, CME 2007 ; Conference date: 23-05-2007 Through 27-05-2007",

}

Liu, Y, Dai, RJ, Qu, F, Meng, W & Deng, Y 2007, Immobilization of trypsin onto artificial membrane for the possible application in the digestion reactor of proteins. in 2007 IEEE/ICME International Conference on Complex Medical Engineering, CME 2007., 4382054, 2007 IEEE/ICME International Conference on Complex Medical Engineering, CME 2007, pp. 1782-1786, 2007 IEEE/ICME International Conference on Complex Medical Engineering, CME 2007, Beijing, China, 23/05/07. https://doi.org/10.1109/ICCME.2007.4382054

Immobilization of trypsin onto artificial membrane for the possible application in the digestion reactor of proteins. / Liu, Yueying; Dai, Rong Ji; Qu, Feng et al.
2007 IEEE/ICME International Conference on Complex Medical Engineering, CME 2007. 2007. p. 1782-1786 4382054 (2007 IEEE/ICME International Conference on Complex Medical Engineering, CME 2007).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

TY - GEN

T1 - Immobilization of trypsin onto artificial membrane for the possible application in the digestion reactor of proteins

AU - Liu, Yueying

AU - Dai, Rong Ji

AU - Qu, Feng

AU - Meng, Weiwei

AU - Deng, Yulin

PY - 2007

Y1 - 2007

N2 - This paper described a novel method to immobilize trypsin on IAM. The immobilization procedures involved the following steps: IAM was packed into the fused silica capillary (400um I.D.×690μm O.D.×5cm length); subsequently, trypsin was dynamically immobilized on IAM by injecting 1mg/ml trypsin aqueous solution using the frontal chromatography. The activity of trypsin-micro-IMERs was on-line measured using BAEE (N-benzoyl-L-arginine ethyl ester) as the substrate of trysin. The results showed that the quantity of product BA and BAEE concentration increased linearly. The results illustrated that the quantity of BA at flow rate of 3 ul/min was obviously larger than that at flow rate of 6 ul/min. The bioactivity of immobilized trypsin was at least 69.3% in 24h.

AB - This paper described a novel method to immobilize trypsin on IAM. The immobilization procedures involved the following steps: IAM was packed into the fused silica capillary (400um I.D.×690μm O.D.×5cm length); subsequently, trypsin was dynamically immobilized on IAM by injecting 1mg/ml trypsin aqueous solution using the frontal chromatography. The activity of trypsin-micro-IMERs was on-line measured using BAEE (N-benzoyl-L-arginine ethyl ester) as the substrate of trysin. The results showed that the quantity of product BA and BAEE concentration increased linearly. The results illustrated that the quantity of BA at flow rate of 3 ul/min was obviously larger than that at flow rate of 6 ul/min. The bioactivity of immobilized trypsin was at least 69.3% in 24h.

KW - BAEE

KW - Dynamica limmobilization

KW - Trypsin-micro-IMERs

UR - http://www.scopus.com/inward/record.url?scp=48149086362&partnerID=8YFLogxK

U2 - 10.1109/ICCME.2007.4382054

DO - 10.1109/ICCME.2007.4382054

M3 - Conference contribution

AN - SCOPUS:48149086362

SN - 1424410789

SN - 9781424410781

T3 - 2007 IEEE/ICME International Conference on Complex Medical Engineering, CME 2007

SP - 1782

EP - 1786

BT - 2007 IEEE/ICME International Conference on Complex Medical Engineering, CME 2007

T2 - 2007 IEEE/ICME International Conference on Complex Medical Engineering, CME 2007

Y2 - 23 May 2007 through 27 May 2007

ER -

Liu Y, Dai RJ, Qu F, Meng W, Deng Y. Immobilization of trypsin onto artificial membrane for the possible application in the digestion reactor of proteins. In 2007 IEEE/ICME International Conference on Complex Medical Engineering, CME 2007. 2007. p. 1782-1786. 4382054. (2007 IEEE/ICME International Conference on Complex Medical Engineering, CME 2007). doi: 10.1109/ICCME.2007.4382054

Immobilization of trypsin onto artificial membrane for the possible application in the digestion reactor of proteins

Abstract

Publication series

Conference

Keywords

Access to Document

Other files and links

Fingerprint

Cite this