Immobilization of BSA on the polymethacrylic acid

Fang Lv, Xiu Jie Liu, Kai Yue Chi, Rong Ji Dai, Yu Lin Deng

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

Abstract

N, N'-Methylenebisacrylamide was used as the crosslinking agent to prepare the polymethacrylic acid and its carboxyl groups were modified by N-(3-Dimethy-laminopropyl)-N'-ethyl carbodiimide hydrochloride and N-Hydroxy succinimide. The ability of immobilizing BSA and the influence of reacting time of carrier were studied. The immobilization amount and the optimal reacting time were 2100mg/g and 90 minutes respectively. The carrier was characterized by infrared detection. The results indicated that the polymethacrylicacid possessed good performance and the ability to immobilized BSA was enhanced after the modification of EDC-NHS.

Original languageEnglish
Title of host publication2013 ICME International Conference on Complex Medical Engineering, CME 2013
Pages501-503
Number of pages3
DOIs
Publication statusPublished - 2013
Event2013 7th ICME International Conference on Complex Medical Engineering, CME 2013 - Beijing, China
Duration: 25 May 201328 May 2013

Publication series

Name2013 ICME International Conference on Complex Medical Engineering, CME 2013

Conference

Conference2013 7th ICME International Conference on Complex Medical Engineering, CME 2013
Country/TerritoryChina
CityBeijing
Period25/05/1328/05/13

Keywords

  • immobilized protein
  • modified carrier
  • polymethacrylicacid

Fingerprint

Dive into the research topics of 'Immobilization of BSA on the polymethacrylic acid'. Together they form a unique fingerprint.

Cite this