Hybridization induced fluorescence turn-on of AIEgen-oligonucleotide conjugates for specific DNA detection

Ruoyu Zhang; Ryan T.K. Kwok; Ben Zhong Tang; Bin Liu

doi:10.1039/c5ra00322a

Hybridization induced fluorescence turn-on of AIEgen-oligonucleotide conjugates for specific DNA detection

Ruoyu Zhang, Ryan T.K. Kwok, Ben Zhong Tang^*, Bin Liu

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

37 Citations (Scopus)

Abstract

A bisazide functionalized fluorogen with aggregation-induced emission characteristics (AIEgen) was conjugated to single-stranded oligonucleotides to yield two-armed AIE probes for specific DNA detection. The probes show low emission in aqueous media, which become highly emissive upon hybridization with their complementary strands. The probes are suitable for homogenous sequence-specific DNA detection and are able to discriminate target sequences with even one-base mutation. The signal output can be further enhanced when two probes hybridize to each other to restrict the free rotation of the incorporated AIEgen.

Original language	English
Pages (from-to)	28332-28337
Number of pages	6
Journal	RSC Advances
Volume	5
Issue number	36
DOIs	https://doi.org/10.1039/c5ra00322a
Publication status	Published - 2015
Externally published	Yes

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abstract = "A bisazide functionalized fluorogen with aggregation-induced emission characteristics (AIEgen) was conjugated to single-stranded oligonucleotides to yield two-armed AIE probes for specific DNA detection. The probes show low emission in aqueous media, which become highly emissive upon hybridization with their complementary strands. The probes are suitable for homogenous sequence-specific DNA detection and are able to discriminate target sequences with even one-base mutation. The signal output can be further enhanced when two probes hybridize to each other to restrict the free rotation of the incorporated AIEgen.",

author = "Ruoyu Zhang and Kwok, {Ryan T.K.} and Tang, {Ben Zhong} and Bin Liu",

note = "Publisher Copyright: {\textcopyright} The Royal Society of Chemistry. 2015.",

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AU - Zhang, Ruoyu

AU - Kwok, Ryan T.K.

AU - Tang, Ben Zhong

AU - Liu, Bin

N1 - Publisher Copyright: © The Royal Society of Chemistry. 2015.

PY - 2015

Y1 - 2015

N2 - A bisazide functionalized fluorogen with aggregation-induced emission characteristics (AIEgen) was conjugated to single-stranded oligonucleotides to yield two-armed AIE probes for specific DNA detection. The probes show low emission in aqueous media, which become highly emissive upon hybridization with their complementary strands. The probes are suitable for homogenous sequence-specific DNA detection and are able to discriminate target sequences with even one-base mutation. The signal output can be further enhanced when two probes hybridize to each other to restrict the free rotation of the incorporated AIEgen.

AB - A bisazide functionalized fluorogen with aggregation-induced emission characteristics (AIEgen) was conjugated to single-stranded oligonucleotides to yield two-armed AIE probes for specific DNA detection. The probes show low emission in aqueous media, which become highly emissive upon hybridization with their complementary strands. The probes are suitable for homogenous sequence-specific DNA detection and are able to discriminate target sequences with even one-base mutation. The signal output can be further enhanced when two probes hybridize to each other to restrict the free rotation of the incorporated AIEgen.

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U2 - 10.1039/c5ra00322a

DO - 10.1039/c5ra00322a

M3 - Article

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SP - 28332

EP - 28337

JO - RSC Advances

JF - RSC Advances

IS - 36

ER -

Hybridization induced fluorescence turn-on of AIEgen-oligonucleotide conjugates for specific DNA detection

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