Determination on high effective strains of degrading nitrotoluene using PCR-DGGE

Yan Hua Yin*, Xue Min Hu, Min Fu

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

To identify the dominant degrading 2, 6-dinitrotoluene(2, 6-DNT) and 4-mononitrotoluene(4-MNT) bacteria in the photocatalysis-anaerobic-aerobic progress, the microbe samples were collected individually from the inlet and outlet of 40 days running anaerobic-aerobic reactor. The measures of polymerase chain reaction (PCR) and denaturing gradient gel electrophoresis (DGGE) were cooperatively used to segregate microbes in the photocatalysis-anaerobic-aerobic microbial community for obtaining the dominant efficiently degrading 2, 6-dinitrotoluene and 4-mononitrotoluene bacteria. It is found that the difference of microbe types is large at different locations in anaerobic reactor, but is less in aerobic reactor. There are five dominant microbes: Delta proteobacterium, Pseudomonas stutzeri, Pseudomonas trivialis, Burkholderia cenocepaciaand Chryseobacterium sp. AKB-2008-VA6 and one unknown microbes. The dominant microbes are facultative anaerobic-aerobic. The water quality is good in 40 days reactor operation.

Original languageEnglish
Pages (from-to)100-104
Number of pages5
JournalBeijing Ligong Daxue Xuebao/Transaction of Beijing Institute of Technology
Volume31
Issue number1
Publication statusPublished - Jan 2011

Keywords

  • Denaturing gradient gel electrophoresis(DGGE)
  • Dominant bacteria
  • Nitrotoluene
  • Polymerase chain reaction(PCR)

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Yin, Y. H., Hu, X. M., & Fu, M. (2011). Determination on high effective strains of degrading nitrotoluene using PCR-DGGE. Beijing Ligong Daxue Xuebao/Transaction of Beijing Institute of Technology, 31(1), 100-104.