Abstract
To identify the dominant degrading 2, 6-dinitrotoluene(2, 6-DNT) and 4-mononitrotoluene(4-MNT) bacteria in the photocatalysis-anaerobic-aerobic progress, the microbe samples were collected individually from the inlet and outlet of 40 days running anaerobic-aerobic reactor. The measures of polymerase chain reaction (PCR) and denaturing gradient gel electrophoresis (DGGE) were cooperatively used to segregate microbes in the photocatalysis-anaerobic-aerobic microbial community for obtaining the dominant efficiently degrading 2, 6-dinitrotoluene and 4-mononitrotoluene bacteria. It is found that the difference of microbe types is large at different locations in anaerobic reactor, but is less in aerobic reactor. There are five dominant microbes: Delta proteobacterium, Pseudomonas stutzeri, Pseudomonas trivialis, Burkholderia cenocepaciaand Chryseobacterium sp. AKB-2008-VA6 and one unknown microbes. The dominant microbes are facultative anaerobic-aerobic. The water quality is good in 40 days reactor operation.
Original language | English |
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Pages (from-to) | 100-104 |
Number of pages | 5 |
Journal | Beijing Ligong Daxue Xuebao/Transaction of Beijing Institute of Technology |
Volume | 31 |
Issue number | 1 |
Publication status | Published - Jan 2011 |
Keywords
- Denaturing gradient gel electrophoresis(DGGE)
- Dominant bacteria
- Nitrotoluene
- Polymerase chain reaction(PCR)