Determination of AKF-PD in whole blood of rat by HPLC-UV

Wei Cao; Lingyun He; Wenfang Liu; Zhizhuang Huang; Zeneng Cheng

doi:10.1016/j.jchromb.2006.01.010

Determination of AKF-PD in whole blood of rat by HPLC-UV

Wei Cao, Lingyun He, Wenfang Liu, Zhizhuang Huang, Zeneng Cheng^*

^*Corresponding author for this work

Central South University

Research output: Contribution to journal › Article › peer-review

15 Citations (Scopus)

Abstract

An HPLC-UV method was developed and validated for the determination of AKF-PD in whole blood of rat. Phenacetin was chosen as the internal standard, and the separation was achieved on a C₁₈ column with methanol and 0.02 M phosphate buffer (pH 3.2) as mobile phase. The obtained calibration graphs were linear (r = 0.9999, n = 9) in the range of 0.203-52.0 μg ml ^-1. The low limit of quantitation was 0.203 μg ml^-1. This method can be used to study the pharmacokinetics of AKF-PD in rat.

Original language	English
Pages (from-to)	257-259
Number of pages	3
Journal	Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
Volume	833
Issue number	2
DOIs	https://doi.org/10.1016/j.jchromb.2006.01.010
Publication status	Published - 3 Apr 2006
Externally published	Yes

Keywords

AKF-PD
HPLC
Pharmacokinetics

Access to Document

10.1016/j.jchromb.2006.01.010

Cite this

@article{5f22c8958df24fa5b5e7dffab2683cff,

title = "Determination of AKF-PD in whole blood of rat by HPLC-UV",

abstract = "An HPLC-UV method was developed and validated for the determination of AKF-PD in whole blood of rat. Phenacetin was chosen as the internal standard, and the separation was achieved on a C18 column with methanol and 0.02 M phosphate buffer (pH 3.2) as mobile phase. The obtained calibration graphs were linear (r = 0.9999, n = 9) in the range of 0.203-52.0 μg ml -1. The low limit of quantitation was 0.203 μg ml-1. This method can be used to study the pharmacokinetics of AKF-PD in rat.",

keywords = "AKF-PD, HPLC, Pharmacokinetics",

author = "Wei Cao and Lingyun He and Wenfang Liu and Zhizhuang Huang and Zeneng Cheng",

year = "2006",

month = apr,

day = "3",

doi = "10.1016/j.jchromb.2006.01.010",

language = "English",

volume = "833",

pages = "257--259",

journal = "Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences",

issn = "1570-0232",

publisher = "Elsevier B.V.",

number = "2",

}

TY - JOUR

T1 - Determination of AKF-PD in whole blood of rat by HPLC-UV

AU - Cao, Wei

AU - He, Lingyun

AU - Liu, Wenfang

AU - Huang, Zhizhuang

AU - Cheng, Zeneng

PY - 2006/4/3

Y1 - 2006/4/3

N2 - An HPLC-UV method was developed and validated for the determination of AKF-PD in whole blood of rat. Phenacetin was chosen as the internal standard, and the separation was achieved on a C18 column with methanol and 0.02 M phosphate buffer (pH 3.2) as mobile phase. The obtained calibration graphs were linear (r = 0.9999, n = 9) in the range of 0.203-52.0 μg ml -1. The low limit of quantitation was 0.203 μg ml-1. This method can be used to study the pharmacokinetics of AKF-PD in rat.

AB - An HPLC-UV method was developed and validated for the determination of AKF-PD in whole blood of rat. Phenacetin was chosen as the internal standard, and the separation was achieved on a C18 column with methanol and 0.02 M phosphate buffer (pH 3.2) as mobile phase. The obtained calibration graphs were linear (r = 0.9999, n = 9) in the range of 0.203-52.0 μg ml -1. The low limit of quantitation was 0.203 μg ml-1. This method can be used to study the pharmacokinetics of AKF-PD in rat.

KW - AKF-PD

KW - HPLC

KW - Pharmacokinetics

UR - http://www.scopus.com/inward/record.url?scp=33645218707&partnerID=8YFLogxK

U2 - 10.1016/j.jchromb.2006.01.010

DO - 10.1016/j.jchromb.2006.01.010

M3 - Article

C2 - 16530492

AN - SCOPUS:33645218707

SN - 1570-0232

VL - 833

SP - 257

EP - 259

JO - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences

JF - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences

IS - 2

ER -

Determination of AKF-PD in whole blood of rat by HPLC-UV

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Cite this