Detection of Fungi from Low-Biomass Spacecraft Assembly Clean Room Aerosols

Ying Zhang; Cong Xin Xin; Lan Tao Zhang; Yu Lin Deng; Xiang Wang; Xiang Yu Chen; Zhao Qian Wang

doi:10.1089/ast.2017.1803

Detection of Fungi from Low-Biomass Spacecraft Assembly Clean Room Aerosols

Ying Zhang^*, Cong Xin Xin, Lan Tao Zhang, Yu Lin Deng, Xiang Wang, Xiang Yu Chen, Zhao Qian Wang

^*Corresponding author for this work

School of Medical and Technology

Research output: Contribution to journal › Article › peer-review

4 Citations (Scopus)

Abstract

Highly sensitive and rapid detection of airborne fungi in space stations is essential to ensure disease prevention and equipment safety. In this study, quantitative loop-mediated isothermal amplification (qLAMP) was used to detect fungi in the aerosol of the low-biomass environment of China's space station assembly clean room (CSSAC). A qLAMP primer set for detecting a wide range of aerosol fungi was developed by aligning 34 sequences of isolated fungal species and 17 space station aerosol-related fungal species. Optimization of sample pretreatment conditions of the LAMP reaction increased the quantitative results by 1.29-1.96 times. The results showed that our qLAMP system had high amplification specificity for fungi, with a quantifiable detection limit as low as 10². The detected fungal biomass in the aerosol of CSSAC was 9.59 × 10²-2.20 × 10⁵ 28S rRNA gene copy numbers/m³. This qLAMP assay may therefore replace traditional colony-forming unit and quantitative PCR methods as an effective strategy for detecting fungi in space stations.

Original language	English
Pages (from-to)	1585-1593
Number of pages	9
Journal	Astrobiology
Volume	18
Issue number	12
DOIs	https://doi.org/10.1089/ast.2017.1803
Publication status	Published - Dec 2018

Keywords

Aerosol fungi
China's space station
Quantitative loopmediated isothermal amplification

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title = "Detection of Fungi from Low-Biomass Spacecraft Assembly Clean Room Aerosols",

abstract = "Highly sensitive and rapid detection of airborne fungi in space stations is essential to ensure disease prevention and equipment safety. In this study, quantitative loop-mediated isothermal amplification (qLAMP) was used to detect fungi in the aerosol of the low-biomass environment of China's space station assembly clean room (CSSAC). A qLAMP primer set for detecting a wide range of aerosol fungi was developed by aligning 34 sequences of isolated fungal species and 17 space station aerosol-related fungal species. Optimization of sample pretreatment conditions of the LAMP reaction increased the quantitative results by 1.29-1.96 times. The results showed that our qLAMP system had high amplification specificity for fungi, with a quantifiable detection limit as low as 102. The detected fungal biomass in the aerosol of CSSAC was 9.59 × 102-2.20 × 105 28S rRNA gene copy numbers/m3. This qLAMP assay may therefore replace traditional colony-forming unit and quantitative PCR methods as an effective strategy for detecting fungi in space stations.",

keywords = "Aerosol fungi, China's space station, Quantitative loopmediated isothermal amplification",

author = "Ying Zhang and Xin, {Cong Xin} and Zhang, {Lan Tao} and Deng, {Yu Lin} and Xiang Wang and Chen, {Xiang Yu} and Wang, {Zhao Qian}",

note = "Publisher Copyright: {\textcopyright} 2018, Mary Ann Liebert, Inc.",

year = "2018",

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doi = "10.1089/ast.2017.1803",

language = "English",

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AU - Zhang, Ying

AU - Xin, Cong Xin

AU - Zhang, Lan Tao

AU - Deng, Yu Lin

AU - Wang, Xiang

AU - Chen, Xiang Yu

AU - Wang, Zhao Qian

PY - 2018/12

Y1 - 2018/12

N2 - Highly sensitive and rapid detection of airborne fungi in space stations is essential to ensure disease prevention and equipment safety. In this study, quantitative loop-mediated isothermal amplification (qLAMP) was used to detect fungi in the aerosol of the low-biomass environment of China's space station assembly clean room (CSSAC). A qLAMP primer set for detecting a wide range of aerosol fungi was developed by aligning 34 sequences of isolated fungal species and 17 space station aerosol-related fungal species. Optimization of sample pretreatment conditions of the LAMP reaction increased the quantitative results by 1.29-1.96 times. The results showed that our qLAMP system had high amplification specificity for fungi, with a quantifiable detection limit as low as 102. The detected fungal biomass in the aerosol of CSSAC was 9.59 × 102-2.20 × 105 28S rRNA gene copy numbers/m3. This qLAMP assay may therefore replace traditional colony-forming unit and quantitative PCR methods as an effective strategy for detecting fungi in space stations.

AB - Highly sensitive and rapid detection of airborne fungi in space stations is essential to ensure disease prevention and equipment safety. In this study, quantitative loop-mediated isothermal amplification (qLAMP) was used to detect fungi in the aerosol of the low-biomass environment of China's space station assembly clean room (CSSAC). A qLAMP primer set for detecting a wide range of aerosol fungi was developed by aligning 34 sequences of isolated fungal species and 17 space station aerosol-related fungal species. Optimization of sample pretreatment conditions of the LAMP reaction increased the quantitative results by 1.29-1.96 times. The results showed that our qLAMP system had high amplification specificity for fungi, with a quantifiable detection limit as low as 102. The detected fungal biomass in the aerosol of CSSAC was 9.59 × 102-2.20 × 105 28S rRNA gene copy numbers/m3. This qLAMP assay may therefore replace traditional colony-forming unit and quantitative PCR methods as an effective strategy for detecting fungi in space stations.

KW - Aerosol fungi

KW - China's space station

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Detection of Fungi from Low-Biomass Spacecraft Assembly Clean Room Aerosols

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Keywords

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