TY - JOUR
T1 - CoO-supported ordered mesoporous carbon nanocomposite based nanozyme with peroxidase-like activity for colorimetric detection of glucose
AU - Guo, Yanyan
AU - Yan, Lu
AU - Zhang, Ruoyu
AU - Ren, Han
AU - Liu, Aihua
N1 - Publisher Copyright:
© 2019 Elsevier Ltd
PY - 2019/6
Y1 - 2019/6
N2 - Nano-based mimetic enzymes have been widely used for catalysis due to their large specific surface area, high stability and activity. Herein a new nanocomposite cobalt oxide (CoO) assembled onto ordered-mesoporous carbon (CoO-OMC) is reported, which shows good peroxidase-like activity upon the oxidation of 3,3′,5,5′-tetramethylbenzidine (TMB) by hydrogen peroxide (H2O2). The apparent maximum velocity (Vmax) and apparent Michaelis–Menten constant (Km) were 2.66 × 10−7 M s−1 and 3.29 mM, respectively. Since the glucose oxidation and TMB oxidation can be bridged by the quantitation of H2O2, therefore, coupled with glucose oxidase, the as-prepared CoO-OMC nanocomposite was applied for the colorimetric detection of glucose in the linear range of 0.1–5.0 mM and the limit of detection of 68 μM glucose. Further, the nanozyme catalyzing platform was used to detect glucose in human serums with good accuracy and reproducibility.
AB - Nano-based mimetic enzymes have been widely used for catalysis due to their large specific surface area, high stability and activity. Herein a new nanocomposite cobalt oxide (CoO) assembled onto ordered-mesoporous carbon (CoO-OMC) is reported, which shows good peroxidase-like activity upon the oxidation of 3,3′,5,5′-tetramethylbenzidine (TMB) by hydrogen peroxide (H2O2). The apparent maximum velocity (Vmax) and apparent Michaelis–Menten constant (Km) were 2.66 × 10−7 M s−1 and 3.29 mM, respectively. Since the glucose oxidation and TMB oxidation can be bridged by the quantitation of H2O2, therefore, coupled with glucose oxidase, the as-prepared CoO-OMC nanocomposite was applied for the colorimetric detection of glucose in the linear range of 0.1–5.0 mM and the limit of detection of 68 μM glucose. Further, the nanozyme catalyzing platform was used to detect glucose in human serums with good accuracy and reproducibility.
KW - Colorimetric assay
KW - CoO-supported ordered mesoporous carbon nanocomposite
KW - Glucose detection
KW - Horseradish peroxidase-like activity
KW - Nanozyme
UR - http://www.scopus.com/inward/record.url?scp=85062913792&partnerID=8YFLogxK
U2 - 10.1016/j.procbio.2019.03.005
DO - 10.1016/j.procbio.2019.03.005
M3 - Article
AN - SCOPUS:85062913792
SN - 1359-5113
VL - 81
SP - 92
EP - 98
JO - Process Biochemistry
JF - Process Biochemistry
ER -