Characterization of molecularly imprinted membrane modified by polystyrene microspheres as porogen for lysozyme adsorption

Wen Tao Zhao; Zhen He Chen; Shu Yang; Xin Zhang; Li Quan Sun; Ai Qin Luo

doi:10.15918/j.tbit1001-0645.2015.supplement1.002

Characterization of molecularly imprinted membrane modified by polystyrene microspheres as porogen for lysozyme adsorption

Wen Tao Zhao, Zhen He Chen, Shu Yang, Xin Zhang, Li Quan Sun, Ai Qin Luo^*

^*Corresponding author for this work

School of Life Science

Research output: Contribution to journal › Article › peer-review

Abstract

A study on the membrane components of rat hippocampus was presented. The isoelectric focusing OFFGEL combing with the reversed-phase liquid chromatography and mass spectrometry was adopted as the major tool to study the separation and enrichment capability of tryptic peptides. The database for annotation, visualization and integrated discovery(DAVID) was used to analyze the acidic proteins we separated based on GO (gene ontology). The results indicate that there are 24 different fractions, each of which is identified by nano-reversed-phase liquid chromatography coupled with quadruple tandem time-of-flight mass spectrophotometer (Q-TOF). About 77% of the identified peptides are found in only one fraction. Moreover, little deviations are observed at low pH value and it has a good focusing effect.

Original language	English
Pages (from-to)	6-10
Number of pages	5
Journal	Beijing Ligong Daxue Xuebao/Transaction of Beijing Institute of Technology
Volume	35
DOIs	https://doi.org/10.15918/j.tbit1001-0645.2015.supplement1.002
Publication status	Published - 1 Jul 2015

Keywords

Fluorescence detection
Lysozyme
Molecularly imprinted polymer membranes
Polystyrene

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10.15918/j.tbit1001-0645.2015.supplement1.002

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title = "Characterization of molecularly imprinted membrane modified by polystyrene microspheres as porogen for lysozyme adsorption",

abstract = "A study on the membrane components of rat hippocampus was presented. The isoelectric focusing OFFGEL combing with the reversed-phase liquid chromatography and mass spectrometry was adopted as the major tool to study the separation and enrichment capability of tryptic peptides. The database for annotation, visualization and integrated discovery(DAVID) was used to analyze the acidic proteins we separated based on GO (gene ontology). The results indicate that there are 24 different fractions, each of which is identified by nano-reversed-phase liquid chromatography coupled with quadruple tandem time-of-flight mass spectrophotometer (Q-TOF). About 77% of the identified peptides are found in only one fraction. Moreover, little deviations are observed at low pH value and it has a good focusing effect.",

keywords = "Fluorescence detection, Lysozyme, Molecularly imprinted polymer membranes, Polystyrene",

author = "Zhao, {Wen Tao} and Chen, {Zhen He} and Shu Yang and Xin Zhang and Sun, {Li Quan} and Luo, {Ai Qin}",

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doi = "10.15918/j.tbit1001-0645.2015.supplement1.002",

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TY - JOUR

T1 - Characterization of molecularly imprinted membrane modified by polystyrene microspheres as porogen for lysozyme adsorption

AU - Zhao, Wen Tao

AU - Chen, Zhen He

AU - Yang, Shu

AU - Zhang, Xin

AU - Sun, Li Quan

AU - Luo, Ai Qin

PY - 2015/7/1

Y1 - 2015/7/1

N2 - A study on the membrane components of rat hippocampus was presented. The isoelectric focusing OFFGEL combing with the reversed-phase liquid chromatography and mass spectrometry was adopted as the major tool to study the separation and enrichment capability of tryptic peptides. The database for annotation, visualization and integrated discovery(DAVID) was used to analyze the acidic proteins we separated based on GO (gene ontology). The results indicate that there are 24 different fractions, each of which is identified by nano-reversed-phase liquid chromatography coupled with quadruple tandem time-of-flight mass spectrophotometer (Q-TOF). About 77% of the identified peptides are found in only one fraction. Moreover, little deviations are observed at low pH value and it has a good focusing effect.

AB - A study on the membrane components of rat hippocampus was presented. The isoelectric focusing OFFGEL combing with the reversed-phase liquid chromatography and mass spectrometry was adopted as the major tool to study the separation and enrichment capability of tryptic peptides. The database for annotation, visualization and integrated discovery(DAVID) was used to analyze the acidic proteins we separated based on GO (gene ontology). The results indicate that there are 24 different fractions, each of which is identified by nano-reversed-phase liquid chromatography coupled with quadruple tandem time-of-flight mass spectrophotometer (Q-TOF). About 77% of the identified peptides are found in only one fraction. Moreover, little deviations are observed at low pH value and it has a good focusing effect.

KW - Fluorescence detection

KW - Lysozyme

KW - Molecularly imprinted polymer membranes

KW - Polystyrene

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U2 - 10.15918/j.tbit1001-0645.2015.supplement1.002

DO - 10.15918/j.tbit1001-0645.2015.supplement1.002

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JO - Beijing Ligong Daxue Xuebao/Transaction of Beijing Institute of Technology

JF - Beijing Ligong Daxue Xuebao/Transaction of Beijing Institute of Technology

ER -

Characterization of molecularly imprinted membrane modified by polystyrene microspheres as porogen for lysozyme adsorption

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