An isothermal nanocalorimetric platform for antimicrobial susceptibility testing based on bacterial metabolic heat measurements

Yang Liu, Thomas Lehnert, Martin A.M. Gijs*

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

Abstract

We present an optimized isothermal nanocalorimetric platform comprising thermopile sensors for performing highly sensitive and rapid bacterial metabolic heat measurements. Based on the specific evolution of the bacterial heat flow curves, different metabolic states could be identified and the metabolic heat generation under various conditions, such as different culture media and temperatures, was studied. We demonstrated the feasibility of fast antimicrobial susceptibility testing and the possibility to determine minimum inhibitor concentrations within a few hours, i.e. on a much shorter timescale than with current clinical setting.

Original languageEnglish
Title of host publication22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018
PublisherChemical and Biological Microsystems Society
Pages717-719
Number of pages3
ISBN (Electronic)9781510897571
Publication statusPublished - 2018
Externally publishedYes
Event22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018 - Kaohsiung, Taiwan, Province of China
Duration: 11 Nov 201815 Nov 2018

Publication series

Name22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018
Volume2

Conference

Conference22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018
Country/TerritoryTaiwan, Province of China
CityKaohsiung
Period11/11/1815/11/18

Keywords

  • Antimicrobial susceptibility
  • Bacteria
  • Metabolic heat
  • Nanocalorimetry

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