A Microfluidic Chip for Circulating Tumor Cells RNA Sequencing at Single Cell Level

Fanghao Shi; Fei Jia; Weikai Zhang; Zewen Wei

doi:10.1109/MEMS46641.2020.9056350

A Microfluidic Chip for Circulating Tumor Cells RNA Sequencing at Single Cell Level

Fanghao Shi, Fei Jia, Weikai Zhang, Zewen Wei^*

^*Corresponding author for this work

School of Medical and Technology

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

Abstract

Here we present an integrated microfluidic chip for single cell RNA sequencing of circulating tumor cells (CTCs). All procedures of single cell RNA sequencing of specific cells in human peripheral blood were integrated in a single microfluidic chip, including blood filtration, CTCs enrichment, single CTC isolation and identification, single cell RNA reverse transcription and cDNA amplification. Our results realized the aim of 'whole blood in, SMART-SEQ II product out' at single CTC level in a microfluidic chip.

Original language	English
Title of host publication	33rd IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2020
Publisher	Institute of Electrical and Electronics Engineers Inc.
Pages	1010-1013
Number of pages	4
ISBN (Electronic)	9781728135809
DOIs	https://doi.org/10.1109/MEMS46641.2020.9056350
Publication status	Published - Jan 2020
Event	33rd IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2020 - Vancouver, Canada Duration: 18 Jan 2020 → 22 Jan 2020

Publication series

Name	Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)
Volume	2020-January
ISSN (Print)	1084-6999

Conference

Conference	33rd IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2020
Country/Territory	Canada
City	Vancouver
Period	18/01/20 → 22/01/20

Keywords

Circulating tumor cells (CTCs)
single cell RNA sequencing

Access to Document

10.1109/MEMS46641.2020.9056350

Cite this

Shi, F., Jia, F., Zhang, W., & Wei, Z. (2020). A Microfluidic Chip for Circulating Tumor Cells RNA Sequencing at Single Cell Level. In 33rd IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2020 (pp. 1010-1013). Article 9056350 (Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS); Vol. 2020-January). Institute of Electrical and Electronics Engineers Inc.. https://doi.org/10.1109/MEMS46641.2020.9056350

Shi, Fanghao ; Jia, Fei ; Zhang, Weikai et al. / A Microfluidic Chip for Circulating Tumor Cells RNA Sequencing at Single Cell Level. 33rd IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2020. Institute of Electrical and Electronics Engineers Inc., 2020. pp. 1010-1013 (Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)).

@inproceedings{1c7d083d62ac4fbf802768e33cff5abf,

title = "A Microfluidic Chip for Circulating Tumor Cells RNA Sequencing at Single Cell Level",

abstract = "Here we present an integrated microfluidic chip for single cell RNA sequencing of circulating tumor cells (CTCs). All procedures of single cell RNA sequencing of specific cells in human peripheral blood were integrated in a single microfluidic chip, including blood filtration, CTCs enrichment, single CTC isolation and identification, single cell RNA reverse transcription and cDNA amplification. Our results realized the aim of 'whole blood in, SMART-SEQ II product out' at single CTC level in a microfluidic chip.",

keywords = "Circulating tumor cells (CTCs), single cell RNA sequencing",

author = "Fanghao Shi and Fei Jia and Weikai Zhang and Zewen Wei",

note = "Publisher Copyright: {\textcopyright} 2020 IEEE.; 33rd IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2020 ; Conference date: 18-01-2020 Through 22-01-2020",

year = "2020",

month = jan,

doi = "10.1109/MEMS46641.2020.9056350",

language = "English",

series = "Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)",

publisher = "Institute of Electrical and Electronics Engineers Inc.",

pages = "1010--1013",

booktitle = "33rd IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2020",

address = "United States",

}

Shi, F, Jia, F, Zhang, W & Wei, Z 2020, A Microfluidic Chip for Circulating Tumor Cells RNA Sequencing at Single Cell Level. in 33rd IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2020., 9056350, Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS), vol. 2020-January, Institute of Electrical and Electronics Engineers Inc., pp. 1010-1013, 33rd IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2020, Vancouver, Canada, 18/01/20. https://doi.org/10.1109/MEMS46641.2020.9056350

A Microfluidic Chip for Circulating Tumor Cells RNA Sequencing at Single Cell Level. / Shi, Fanghao; Jia, Fei; Zhang, Weikai et al.
33rd IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2020. Institute of Electrical and Electronics Engineers Inc., 2020. p. 1010-1013 9056350 (Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS); Vol. 2020-January).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

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AU - Wei, Zewen

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N2 - Here we present an integrated microfluidic chip for single cell RNA sequencing of circulating tumor cells (CTCs). All procedures of single cell RNA sequencing of specific cells in human peripheral blood were integrated in a single microfluidic chip, including blood filtration, CTCs enrichment, single CTC isolation and identification, single cell RNA reverse transcription and cDNA amplification. Our results realized the aim of 'whole blood in, SMART-SEQ II product out' at single CTC level in a microfluidic chip.

AB - Here we present an integrated microfluidic chip for single cell RNA sequencing of circulating tumor cells (CTCs). All procedures of single cell RNA sequencing of specific cells in human peripheral blood were integrated in a single microfluidic chip, including blood filtration, CTCs enrichment, single CTC isolation and identification, single cell RNA reverse transcription and cDNA amplification. Our results realized the aim of 'whole blood in, SMART-SEQ II product out' at single CTC level in a microfluidic chip.

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T3 - Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)

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PB - Institute of Electrical and Electronics Engineers Inc.

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ER -

Shi F, Jia F, Zhang W, Wei Z. A Microfluidic Chip for Circulating Tumor Cells RNA Sequencing at Single Cell Level. In 33rd IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2020. Institute of Electrical and Electronics Engineers Inc. 2020. p. 1010-1013. 9056350. (Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)). doi: 10.1109/MEMS46641.2020.9056350

A Microfluidic Chip for Circulating Tumor Cells RNA Sequencing at Single Cell Level

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