A metabolic labeling way to in situ fabricate bacterial FRET Platform for innate immune defence molecule

Zhijun Zhang; Qinyu Han; Jun Wei Lau; Zhimin Wang; Ming Hu; Hao Qiu; Thang Cong Do; Bengang Xing

doi:10.1016/j.snb.2021.130913

A metabolic labeling way to in situ fabricate bacterial FRET Platform for innate immune defence molecule

Zhijun Zhang^*, Qinyu Han, Jun Wei Lau, Zhimin Wang, Ming Hu, Hao Qiu, Thang Cong Do, Bengang Xing^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

5 Citations (Scopus)

Abstract

A simple and unique bacterial fluorescence resonance energy transfer (FRET) platform is developed through metabolic biosynthetic pathways for the sensitive and ratiometric detection of innate immune defence molecule. Using this bacterial FRET platform, immune molecule lysozyme could be sensitively monitored in buffer, in serum, and even in the secretion of infected immune cells. As an important defensive molecule of the innate immune system, lysozyme not only plays a significant role in mediating protection against microbial invasion, but also acts as a significant biomarker of leukemia, tuberculosis, meningitis and renal diseases. Considering the importance of perceiving lysozyme activity, this work sheds a light on the access of host's immune response of bacterial infection, as well as provides valuable guidance for the treatment of bacterial infection related diseases.

Original language	English
Article number	130913
Journal	Sensors and Actuators, B: Chemical
Volume	350
DOIs	https://doi.org/10.1016/j.snb.2021.130913
Publication status	Published - 1 Jan 2022
Externally published	Yes

Keywords

Bacteria-based sensor
Bacterial infection
FRET
Fluorescence labeling
Lysozyme
Metabolic labeling

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.snb.2021.130913

Cite this

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title = "A metabolic labeling way to in situ fabricate bacterial FRET Platform for innate immune defence molecule",

abstract = "A simple and unique bacterial fluorescence resonance energy transfer (FRET) platform is developed through metabolic biosynthetic pathways for the sensitive and ratiometric detection of innate immune defence molecule. Using this bacterial FRET platform, immune molecule lysozyme could be sensitively monitored in buffer, in serum, and even in the secretion of infected immune cells. As an important defensive molecule of the innate immune system, lysozyme not only plays a significant role in mediating protection against microbial invasion, but also acts as a significant biomarker of leukemia, tuberculosis, meningitis and renal diseases. Considering the importance of perceiving lysozyme activity, this work sheds a light on the access of host's immune response of bacterial infection, as well as provides valuable guidance for the treatment of bacterial infection related diseases.",

keywords = "Bacteria-based sensor, Bacterial infection, FRET, Fluorescence labeling, Lysozyme, Metabolic labeling",

author = "Zhijun Zhang and Qinyu Han and Lau, {Jun Wei} and Zhimin Wang and Ming Hu and Hao Qiu and Do, {Thang Cong} and Bengang Xing",

note = "Publisher Copyright: {\textcopyright} 2021 Elsevier B.V.",

year = "2022",

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doi = "10.1016/j.snb.2021.130913",

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TY - JOUR

T1 - A metabolic labeling way to in situ fabricate bacterial FRET Platform for innate immune defence molecule

AU - Zhang, Zhijun

AU - Han, Qinyu

AU - Lau, Jun Wei

AU - Wang, Zhimin

AU - Hu, Ming

AU - Qiu, Hao

AU - Do, Thang Cong

AU - Xing, Bengang

PY - 2022/1/1

Y1 - 2022/1/1

N2 - A simple and unique bacterial fluorescence resonance energy transfer (FRET) platform is developed through metabolic biosynthetic pathways for the sensitive and ratiometric detection of innate immune defence molecule. Using this bacterial FRET platform, immune molecule lysozyme could be sensitively monitored in buffer, in serum, and even in the secretion of infected immune cells. As an important defensive molecule of the innate immune system, lysozyme not only plays a significant role in mediating protection against microbial invasion, but also acts as a significant biomarker of leukemia, tuberculosis, meningitis and renal diseases. Considering the importance of perceiving lysozyme activity, this work sheds a light on the access of host's immune response of bacterial infection, as well as provides valuable guidance for the treatment of bacterial infection related diseases.

AB - A simple and unique bacterial fluorescence resonance energy transfer (FRET) platform is developed through metabolic biosynthetic pathways for the sensitive and ratiometric detection of innate immune defence molecule. Using this bacterial FRET platform, immune molecule lysozyme could be sensitively monitored in buffer, in serum, and even in the secretion of infected immune cells. As an important defensive molecule of the innate immune system, lysozyme not only plays a significant role in mediating protection against microbial invasion, but also acts as a significant biomarker of leukemia, tuberculosis, meningitis and renal diseases. Considering the importance of perceiving lysozyme activity, this work sheds a light on the access of host's immune response of bacterial infection, as well as provides valuable guidance for the treatment of bacterial infection related diseases.

KW - Bacteria-based sensor

KW - Bacterial infection

KW - FRET

KW - Fluorescence labeling

KW - Lysozyme

KW - Metabolic labeling

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U2 - 10.1016/j.snb.2021.130913

DO - 10.1016/j.snb.2021.130913

M3 - Article

AN - SCOPUS:85117361455

SN - 0925-4005

VL - 350

JO - Sensors and Actuators, B: Chemical

JF - Sensors and Actuators, B: Chemical

M1 - 130913

ER -

A metabolic labeling way to in situ fabricate bacterial FRET Platform for innate immune defence molecule

Abstract

Keywords

UN SDGs

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