A high performance continuous electroporation chip

Zewen Wei, Huang Huang, Mengxi Wu, Zicai Liang, Wei Wang, Zhihong Li*

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

1 Citation (Scopus)

Abstract

Electroporation is an effective method for delivery of biological components into mammalian cells and is of great importance for modern life science. Here we report a novel continuous electroporation chip with great performance. Integration of the microfluid channel, micromachined Au electrodes and hydrodynamic focusing enables high-throughput and high-efficiency electroporation. Using the standard expression cell line HEK-293 and expression vector pEGFP-C3, we realized excellent transfection rate (90%), cell viability (60%) and cell treating speed (1.5×10 cells per minute), all much higher than those reported in literature.

Original languageEnglish
Title of host publication14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010
Pages1622-1624
Number of pages3
Publication statusPublished - 2010
Externally publishedYes
Event14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010 - Groningen, Netherlands
Duration: 3 Oct 20107 Oct 2010

Publication series

Name14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010
Volume3

Conference

Conference14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010
Country/TerritoryNetherlands
CityGroningen
Period3/10/107/10/10

Keywords

  • Electroporation
  • High throughput
  • Hydrodynamic focusing
  • Microfluid

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