A flow-through electroporation chip integrated with viable cell sorting based on dielectrophoresis

Xueming Li; Zewen Wei; Deyao Zhao; Hao Yan; Zicai Liang; Zhihong Li

doi:10.1109/MEMSYS.2012.6170353

A flow-through electroporation chip integrated with viable cell sorting based on dielectrophoresis

Xueming Li, Zewen Wei, Deyao Zhao, Hao Yan, Zicai Liang, Zhihong Li^*

^*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

3 Citations (Scopus)

Abstract

Flow-through electroporation on a chip is a promising technique to introduce molecules into cells in applications of biological research, drug delivery and gene therapy. During the electroporation process, the electrical field also causes large amount of non-viable cells, which significantly influence the following biological procedure. Off-chip separation of the variable cells increases experimental complexity dramatically and results in further cell death. In this paper, we have developed a flow-through electroporation chip, in which dielectrophoresis (DEP) is employed to sort the viable cells and non-viable cells on chip. For the standard expression cell line HEK-293a (Human embryonic kidney cells), the ratio of the viable cells in the sorted sample was increased to 90% from 20% in the as-electroporated sample.

Original language	English
Title of host publication	2012 IEEE 25th International Conference on Micro Electro Mechanical Systems, MEMS 2012
Pages	993-996
Number of pages	4
DOIs	https://doi.org/10.1109/MEMSYS.2012.6170353
Publication status	Published - 2012
Externally published	Yes
Event	2012 IEEE 25th International Conference on Micro Electro Mechanical Systems, MEMS 2012 - Paris, France Duration: 29 Jan 2012 → 2 Feb 2012

Publication series

Name	Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)
ISSN (Print)	1084-6999

Conference

Conference	2012 IEEE 25th International Conference on Micro Electro Mechanical Systems, MEMS 2012
Country/Territory	France
City	Paris
Period	29/01/12 → 2/02/12

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10.1109/MEMSYS.2012.6170353

Cite this

Li, X., Wei, Z., Zhao, D., Yan, H., Liang, Z., & Li, Z. (2012). A flow-through electroporation chip integrated with viable cell sorting based on dielectrophoresis. In 2012 IEEE 25th International Conference on Micro Electro Mechanical Systems, MEMS 2012 (pp. 993-996). Article 6170353 (Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)). https://doi.org/10.1109/MEMSYS.2012.6170353

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title = "A flow-through electroporation chip integrated with viable cell sorting based on dielectrophoresis",

abstract = "Flow-through electroporation on a chip is a promising technique to introduce molecules into cells in applications of biological research, drug delivery and gene therapy. During the electroporation process, the electrical field also causes large amount of non-viable cells, which significantly influence the following biological procedure. Off-chip separation of the variable cells increases experimental complexity dramatically and results in further cell death. In this paper, we have developed a flow-through electroporation chip, in which dielectrophoresis (DEP) is employed to sort the viable cells and non-viable cells on chip. For the standard expression cell line HEK-293a (Human embryonic kidney cells), the ratio of the viable cells in the sorted sample was increased to 90% from 20% in the as-electroporated sample.",

author = "Xueming Li and Zewen Wei and Deyao Zhao and Hao Yan and Zicai Liang and Zhihong Li",

year = "2012",

doi = "10.1109/MEMSYS.2012.6170353",

language = "English",

isbn = "9781467303248",

series = "Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)",

pages = "993--996",

booktitle = "2012 IEEE 25th International Conference on Micro Electro Mechanical Systems, MEMS 2012",

note = "2012 IEEE 25th International Conference on Micro Electro Mechanical Systems, MEMS 2012 ; Conference date: 29-01-2012 Through 02-02-2012",

}

Li, X, Wei, Z, Zhao, D, Yan, H, Liang, Z & Li, Z 2012, A flow-through electroporation chip integrated with viable cell sorting based on dielectrophoresis. in 2012 IEEE 25th International Conference on Micro Electro Mechanical Systems, MEMS 2012., 6170353, Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS), pp. 993-996, 2012 IEEE 25th International Conference on Micro Electro Mechanical Systems, MEMS 2012, Paris, France, 29/01/12. https://doi.org/10.1109/MEMSYS.2012.6170353

A flow-through electroporation chip integrated with viable cell sorting based on dielectrophoresis. / Li, Xueming; Wei, Zewen; Zhao, Deyao et al.
2012 IEEE 25th International Conference on Micro Electro Mechanical Systems, MEMS 2012. 2012. p. 993-996 6170353 (Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

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AU - Li, Zhihong

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AB - Flow-through electroporation on a chip is a promising technique to introduce molecules into cells in applications of biological research, drug delivery and gene therapy. During the electroporation process, the electrical field also causes large amount of non-viable cells, which significantly influence the following biological procedure. Off-chip separation of the variable cells increases experimental complexity dramatically and results in further cell death. In this paper, we have developed a flow-through electroporation chip, in which dielectrophoresis (DEP) is employed to sort the viable cells and non-viable cells on chip. For the standard expression cell line HEK-293a (Human embryonic kidney cells), the ratio of the viable cells in the sorted sample was increased to 90% from 20% in the as-electroporated sample.

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Li X, Wei Z, Zhao D, Yan H, Liang Z, Li Z. A flow-through electroporation chip integrated with viable cell sorting based on dielectrophoresis. In 2012 IEEE 25th International Conference on Micro Electro Mechanical Systems, MEMS 2012. 2012. p. 993-996. 6170353. (Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)). doi: 10.1109/MEMSYS.2012.6170353

A flow-through electroporation chip integrated with viable cell sorting based on dielectrophoresis

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